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Transformed cells after senescence give rise to more severe tumor phenotypes than transformed non-senescent cells

ABSTRACT: Transformed cells after senescence give rise to more severe tumor phenotypes than transformed non-senescent cells

PROVIDER: PRJNA863600 | ENA |

REPOSITORIES: ENA

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Transformed cells after senescence give rise to more severe tumor phenotypes than transformed non-senescent cells

Project description:Oncogenic stress-induced senescence initially inhibits tumor initiation by blocking proliferation. If these cells are not eliminated they may resume proliferation upon loss-of-tumor suppressors, and be at risk of transformation. During tumor formation, depending on the sequence of events of gain-of-oncogenes and/or loss-of-tumor suppressors, cancer cells may emerge from senescent cells. The goal of this study is to determine if transformed cells after senescence (TS) display more aggressive tumorigenic features, with a greater capacity to migrate and a higher resistance to anti-tumoral drugs than cells having undergone transformation without senescence. Here, we modeled cell transformation using mouse embryonic fibroblasts (MEFs) subjected to an inverse sequence of events: i) gain-of-RasV12 oncogene and loss-of-p53 tumor suppressor, leading to transformed cells after senescence (TS), or ii) the opposite, resulting in transformed (T) non-senescent cells.

2022-07-30 | GSE210060 | GEO

Human hepatocytes can give rise to intrahepatic cholangiocarcinomas

Project description:Human hepatocytes can give rise to intrahepatic cholangiocarcinomas

| PRJNA915042 | ENA

Autoimmunity and infection give rise to distict inflammatory Th17 subsets

Project description:Autoimmunity and infection give rise to distict inflammatory Th17 subsets

| PRJNA982096 | ENA

Bone marrow-derived monocytes give rise to self-renewing and fully differentiated Kupffer cells

Project description:Self-renewing tissue-resident macrophages are thought to be exclusively derived from embryonic progenitors. However, whether circulating monocytes can also give rise to such macrophages has not been formally investigated. Here we use a new model of diphtheria toxin-mediated depletion of liver-resident Kupffer cells to generate niche availability and show that circulating monocytes engrafted in the liver, gradually adopt the transcriptional profile of their depleted counterparts and become long-lived self-renewing cells. Underlining the physiological relevance of our findings, circulating monocytes also contribute to the expanding pool of macrophages in the liver shortly after birth, when macrophage niches become available during normal organ growth. Thus, like embryonic precursors, monocytes can and do give rise to self-renewing tissue-resident macrophages if the niche is available to them. Clec4F+ Kupffer cells were isolated and sorted from livers from adult WT mice or KC-DTR or KC-DTR littermate control mice +/- 50ng DT at indicated timepoints. 19 samples (arrays) in total. RNA was isolated, amplified with Nugene pico kit, converted to cDNA and then hybridised on Affymetrix GeneChip Mouse Gene 1.0 ST Arrays.

2015-11-21 | E-GEOD-75225 | biostudies-arrayexpress

Bone marrow-derived monocytes give rise to self-renewing and fully differentiated Kupffer cells

2015-11-21 | GSE75225 | GEO

Neural plate progenitors give rise to both anterior and posterior pituitary cells

Project description:Neural plate progenitors give rise to both anterior and posterior pituitary cells

| PRJNA838481 | ENA

Autoimmunity and infection give rise to distict inflammatory Th17 subsets [bulk RNA-seq]

Project description:Autoimmunity and infection give rise to distict inflammatory Th17 subsets [bulk RNA-seq]

| PRJNA982103 | ENA

Gramd2+ alveolar type I cells can give rise to Kras-induced lung adenocarcinoma

Project description:Gramd2+ alveolar type I cells can give rise to Kras-induced lung adenocarcinoma

| PRJNA891038 | ENA

Active Promoters give rise to false positive 'Phantom Peaks' in ChIP-Seq experiments

Project description:We applied ChIP-seq to map the chromosomal binding sites for two nucleosome remodeling complexes containing the ATPase ISWI, ACF and RSF, in Drosophila embryos. Employing a panel of polyclonal and monoclonal antibodies directed against their signature subunits, ACF1 and RSF1, robust profiles were obtained indicating that both remodelers co-occupied a large set of active promoters. For further validation we repeated the mapping using chromatin of mutant embryos that do not express ACF1 or RSF1. Surprisingly, the ChIP-seq profiles were unchanged, suggesting that they were not due to specific immunoprecipitation. Conservative analysis lists about 3000 chromosomal loci, mostly active promoters that are prone to non-specific enrichment in ChIP and give rise to ‘Phantom Peaks’. These peaks are not obtained with pre-immune serum and are not prominent in input chromatin. Examination of various ACF1 and RSF1 antibodies in Drosophila melanogaster embryos which are wildtype or mutant for the antibody targets.

2015-07-10 | E-GEOD-67323 | biostudies-arrayexpress

Autoimmunity and infection give rise to distict inflammatory Th17 subsets [single-cell RNA-seq]

Project description:Autoimmunity and infection give rise to distict inflammatory Th17 subsets [single-cell RNA-seq]

| PRJNA982101 | ENA