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De-differentiation and Proliferation of Artery Endothelial Cells Drive Coronary Collateral Development

ABSTRACT: De-differentiation and Proliferation of Artery Endothelial Cells Drive Coronary Collateral Development

PROVIDER: PRJNA865170 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20749409_1.fastq.gz	Fastqsanger.gz
	SRR20749409_2.fastq.gz	Fastqsanger.gz
	SRR20749410_1.fastq.gz	Fastqsanger.gz
	SRR20749410_2.fastq.gz	Fastqsanger.gz
	SRR20749411_1.fastq.gz	Fastqsanger.gz

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De-differentiation and Proliferation of Artery Endothelial Cells Drive Coronary Collateral Development

Project description:We have earlier observed that upon myocardial infarction, pre-existing coronary arteries give rise to new collateral arteries via Artery Reassembly (AR) in neonatal mice, but not in adults. To understand this age dependent phenomenon, Gja5 (Cx40), an established artery endothelial cell marker, was used to lineage trace artery cells with TdTomato expression. For the neonatal mice, Tamoxifen was induced at postnatal (P)0, Myocardial Infarction (MI)/sham was performed at P2 and hearts were harvested at either P4 or P5. Whereas, for the adults, mice aged ~2months were used. Tamoxifen was induced at Day (D)0, MI/sham was performed at D2 and hearts were harvested at either D9 or D11. Ventricles from hearts were digested and FACS sorted for DAPI-, Ter199- and TdTomato+ cells.

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Transcription profiling of human coronary arteries from patients with and without collateral artery vessels to identify novel markers of coronary collateralization

Project description:Evaluation of transcriptional regulation in Coronary artery disease patients with and without collateral artery vessels.

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Circulating Cells in Coronary Collateral Artery Growth

Project description:Background: Collateral artery growth, also termed arteriogenesis, occurs upon narrowing or occlusion of a major artery. To date, attempts to stimulate arteriogenesis in patients for therapeutic purposes have not been successful. Experimental models showed that circulating cells orchestrate arteriogenesis. In humans, a large heterogeneity exists in the arteriogenic response. We hypothesized that good arteriogenic responders (GARs) and bad arteriogenic responders (BARs) differ in gene expression profiles of circulating cells, thereby disclosing potential new therapeutic strategies for the stimulation of arteriogenesis. Methods: A total of 45 patients scheduled for percutaneous coronary intervention (PCI) for single-vessel coronary artery disease underwent intracoronary measurements of collateral flow index (CFI) to distinguish between GARs (CFI>0.21) and BARs (CFI≤0.21). Monocytes and CD34+ stem cells were collected from peripheral blood. A fraction of monocytes was further processed to obtain stimulated monocytes and macrophages. Whole genome transcriptome analysis was performed on all four cell types. Results: LPS-stimulated monocytes showed 244 genes differentially expressed (false discovery rate < 0.05) between GARs and BARs. Macrophage gene expression correlated with stimulated monocytes, while resting monocytes and stem cells revealed no differential gene expression. Stimulated monocytes from GARs showed a strongly attenuated response in several interferon- and apoptosis-related genes, which was corroborated at the protein level. Conclusions Circulating cells from GARs and BARs distinctively differ in their gene expression profiles upon stimulation. The reduced expression of interferon and apoptosis genes in GARs may lead to novel therapeutic approaches for the stimulation of collateral artery growth. Keywords: disease state analysis, stress response analysis, natural defense mechanism analysis

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Circulating Cells in Coronary Collateral Artery Growth II

Project description:In the present study, we performed transcriptome expression analyses in three independent peripheral blood-derived monocyte subpopulations from patients with chronic coronary occlusions (CTO) and tested for arteriogenesis. Whole-genome mRNA expression analyses were performed on these three monocyte subpopulations, namely: (1) unstimulated-, (2) 3 hours LPS-stimulated-, (3) monocyte-derived macrophages. Whole-genome mRNA expression analysis amongst others confirmed increased expression of IFNbeta-regulated genes in patients with insufficient coronary collateralization (collateral flow index (CFI) ≤0.37 “nonresponders”), compared with patients having sufficient collateralization (CFI>0.37 “responders”).

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LncRNA expression profiling for 6 human monocytes samples from Coronary Artery Disease patients and non Coronary Artery Disease patients

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Project description:The human LncRNA microarray analysis of the 6 monocytes samples from Coronary Artery Disease patients and non Coronary Artery Disease

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LncRNA expression profiling for 6 human plasma samples from Coronary Artery Disease patients and non Coronary Artery Disease patients

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