Project description:Eggs and juveniles of NWE-Mc31 from Meloidogyne chitwoodi were collected and total RNA was isolated as described in the sample collection protocol. The quality of both RNA isolations was RIN 10 (RNA Integrity Number). mRNA sequences were generated using the TruSeq RNA stranded polyA protocol of Useq (Utrecht Sequencing Facility, Utrecht, The Netherlands.
Project description:High-coverage whole genome sequencing of 11 Brazilian isolates of the root-knot nematode Meloidogyne incognita, presenting different host plant preferences and different geographical origins. Four M. incognita host races had been proposed in the past, based on host (in)compatibility on four different plant strains. The objective was to assess whether genomic variations (SNP) correlate with host range compatibility, geographical origin and host plant of origin.
