Similar Datasets
Project description:Deep sequencing of mRNA from the halictid Part of the Lasioglossum albipes WGS project (BioProject ID: PRJNA174755) Analysis of ploy(A)+ RNA of different specimens:whole body from the halictid (Lasioglossum albipes)
2012-10-02 | E-GEOD-41247 | biostudies-arrayexpress
Project description:Deep sequencing of mRNA from the halictid Part of the Lasioglossum albipes WGS project (BioProject ID: PRJNA174755)
2012-10-02 | GSE41247 | GEO
Project description:Comparative transcriptomics of pheromone biosynthesis and chemoreception among the halictid sweat bees.
| PRJNA629833 | ENA
Project description:Honeybees are very important eusocial insects and are involved in the pollination of many plants. Queen bees and worker bees develop from the same fertilized eggs, and are thus genetically identical despite their substantial behavioural and physiological differences. The mechanism governing developmental differences between worker and queen bees has always attracted much interest. While there are several reports on mRNA expression related to caste differentiation, no systematic investigation of small RNAs has thus far been carried out. Results: Using deep sequencing we systematically profiled small RNA expression in 4th-6th day worker larvae and queen larvae (the critical stages at which the fates of workers and queens are determined), and found that 38 miRNAs were differentially expressed between worker and queen larvae. In addition, 639 mature miRNA candidates were identified in our work for the first time, of which, 526 were expressed only in workers (318) or queens (208). Conclusion: We present the first profile of honeybee small RNAs and explore the mechanism of caste differentiation between worker and queen bees. Caste-specific expression patterns and large discrepancies in small RNA profiles between worker and queen bees indicate that small RNAs may be related to the differential development of worker and queen bee larvae. Results presented here will make a valuable contribution to understanding the caste switch between worker and queen bees.
2014-07-28 | GSE52630 | GEO
Project description:Experimental infection of (2 days old) adult honey bee workers (30 bees per replicates, 3 replicates per treatments, from 3 different colonies (one colony per cage for each treatment)) with 10^9 genome equivalent of Black Queen Cell Virus (BQCV) in 10µl of sugar solution and/or 10^5 fresh Nosema ceranae spores (control bees were given a similar bee extract in PBS, without pathogen). Bees were kept in cages of 30 bees in incubator (30°C/50%RH). At day 13 p.i., bees were flash frozen, and stored at -80°C.
2016-09-06 | GSE81664 | GEO
Project description:Seeking to further characterize the effect of Halofuginone in honey bees and to better understand cellular stress pathways in this species more generally, we used RNAseq to identify novel changes in gene expression after its ingestion and observed a striking induction of genes involved in ribosome biogenesis, translation, tRNA synthesis, and ribosome-associated quality control (RQC). Feeding bees cycloheximide (CHX) to halt ribosome translocation also induced all tested target genes, suggesting that this gene expression program could be induced by ribosome stalling in addition to tRNA-synthetase inhibition. Only a subset of these ribosome biogenesis genes are upregulated by UPR induction, suggesting that the ISR induces overlapping but distinct responses depending on the mode of activation and cross-talk with other cellular signaling pathways.
2021-10-22 | GSE165411 | GEO
Project description:The microsporidia Nosema ceranae are intracellular parasites that proliferate in the midgut epithelial cells of honey bees (Apis mellifera). To analyze the pathological effects of those microsporidia, we orally infected honey bee workers 7 days after their emergence. Bees were flash frozen 15 days after the infection. Then, the effects on the gut ventriculi were analyzed and compared to non-infected (control) bees.
2012-05-23 | GSE25455 | GEO