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Effect of LL-2 conditional medium on bone marrow-derived macrophages

ABSTRACT: Effect of LL-2 conditional medium on bone marrow-derived macrophages

PROVIDER: PRJNA867394 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20964678_1.fastq.gz	Fastqsanger.gz
	SRR20964678_2.fastq.gz	Fastqsanger.gz
	SRR20964679_1.fastq.gz	Fastqsanger.gz
	SRR20964679_2.fastq.gz	Fastqsanger.gz
	SRR20964680_1.fastq.gz	Fastqsanger.gz

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Project description:The goal of this study is to investigate the presence of novel small RNAs in apoptotic macrophage Method: cultured mouse (bl6 line) bone marrow were differentiated in for 7 days in conditioned medium, in preence of Macrophage-Colony Stimulating Factor (M-CSF). M-CSF also stimulates the survive of macrophages by activating PI3K/AKT pathway. In accordance to previous publication (Lombardo, E., Alvarez-Barrientos, A., Maroto, B., Bosca, L., and Knaus, U.G, 2007, TLR4-mediated survival of macrophages is MyD88 dependent and requires TNF-alpha autocrine signalling, J Immunol 178, 3731-3739) apoptosis was induced in culturaed bone marrow-derived macrophages upon 36 hr of M-CSF withdrawal. Total RNA was isolated and a small RNA library was made using "Small RNA sequencing kit" (Illumina), according to the manufacturer’s instructions.

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