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Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity

ABSTRACT: Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity

PROVIDER: PRJNA867742 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20995800.fastq.gz	Fastqsanger.gz
	SRR20995801.fastq.gz	Fastqsanger.gz
	SRR20995803.fastq.gz	Fastqsanger.gz
	SRR20995804.fastq.gz	Fastqsanger.gz
	SRR20995805.fastq.gz	Fastqsanger.gz

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Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity [ChIP-seq]

Project description:Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity [ChIP-seq]

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Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity [TT-seq]

Project description:Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity [TT-seq]

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Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity [DRB/TT-seq]

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Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity [TT-seq]

Project description:To investigate the role of catalytic subunit of the Integrator complex, INTS11, during the transcription cycle, we established U2OS cell line in which INTS11 has been knock down by siRNA.

2023-07-27 | GSE210877 | GEO

Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity [ChIP-seq]

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Human Integrator provides a quality checkpoint during elongation to facilitate RNA polymerase II processivity

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-07-27 | GSE210880 | GEO

Spt4 promotes Pol I processivity and transcription elongation

Project description:Spt4 promotes Pol I processivity and transcription elongation

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Regulation of RNA polymerase II processivity by Spt5 is restricted to a narrow window in elongation

Project description:Spt5 is a highly conserved RNA polymerase II (Pol II)-associated pausing and elongation factor. However, its impact on global elongation and Pol II processivity in mammalian cells has not been clarified. Here, we show that depleting Spt5 in mouse embryonic fibroblasts (MEFs) does not cause global elongation defects or decreased elongation rates. Instead, in the absence of Spt5, a fraction of Pol II molecules are dislodged during elongation thus decreasing the number of Pol II complexes that complete the transcription cycle. Most strikingly, this decrease is restricted to a narrow window between 15-20 kb from the promoter, a distance which coincides with the stage where accelerating Pol II attains maximum elongation speed and processivity. Consequently, long genes show a greater dependency on Spt5 for optimal elongation efficiency and overall gene expression than short genes. We propose that an important role of Spt5 in mammalian elongation is to promote the processivity of those Pol II complexes that are transitioning towards maximum elongation speed 15-20 kb from the promoter.

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The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts

Project description:Transcription by RNA polymerase II (RNAPII) is pervasive in the human genome. However, the mechanisms controlling transcription at promoters and enhancers remain enigmatic. Here, we demonstrate that Integrator subunit 11 (INTS11), the catalytic subunit of the Integrator complex, regulates transcription at these loci through its endonuclease activity. Promoters of genes require INTS11 to cleave nascent transcripts associated with paused RNAPII and induce their premature termination in the proximity of the +1 nucleosome. The turnover of RNAPII permits the subsequent recruitment of an elongation-competent RNAPII complex, leading to productive elongation. In contrast, enhancers require INTS11 catalysis not to evict paused RNAPII but rather to terminate enhancer RNA transcription beyond the +1 nucleosome. These findings are supported by the differential occupancy of negative elongation factor (NELF), SPT5, and tyrosine-1-phosphorylated RNAPII. This study elucidates the role of Integrator in mediating transcriptional elongation at human promoters through the endonucleolytic cleavage of nascent transcripts and the dynamic turnover of RNAPII.

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