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Differential expression of miRNAs and mRNAs in Moritella viscosa challenge Atlantic salmon

ABSTRACT: Differential expression of miRNAs and mRNAs in Moritella viscosa challenge Atlantic salmon

PROVIDER: PRJNA868593 | ENA |

REPOSITORIES: ENA

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Differential expression of miRNAs and mRNAs in Moritella viscosa challenge Atlantic salmon

Project description:Moritella viscosa is a bacterial pathogen causing winter-ulcer disease in Atlantic salmon. The lesions on affected fish lead to increased mortality, decreased fish welfare, and inferior meat quality in farmed salmon. MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional regulation by guiding the miRNA induced silencing complex to specific mRNA transcripts (target genes). The goal of this study was to identify miRNAs responding to Moritella viscosa in salmon by investigating miRNA expression in head-kidney and the muscle/skin from lesion sites caused by the pathogen. Protein coding gene expression was investigated by microarray analysis in the same materials.

2022-08-14 | GSE211004 | GEO

Responses of Atlantic salmon to vaccination and challenge with salmon alphavirus (SAV) and Moritella viscosa

Project description:Salmon alphavirus (SAV) and Moritella viscosa causing respectively pancreatic disease and winter ulcer are among the most important pathogens threatening Atlantic salmon aquaculture. Fish is protected by vaccination with different rate of success. Here, responses to vaccination were assessed followed with pathogen challenges of vaccinated salmon and saline injected control.

2021-04-23 | GSE173130 | GEO

Responses to Moritella viscosa in skin and spleen of Atlantic salmon

Project description:Bacterial pathogen Moritella viscosa, the causative agent of winter ulcer, causes heavy losses in Atlantic salmon aquaculture. The study compared responses in salmon reared under normal condition (G100) and fish exposed to hypoxia - 60% saturation of dissolved oxygen - at early life (G60). G60 showed lower survival after challenge. Analyses were performed in the most affected tissues: skin and spleen

2021-04-09 | GSE171693 | GEO

Responses of Atlantic salmon to vaccination and challenge with salmon alphavirus (SAV) and Moritella viscosa

Project description:Responses of Atlantic salmon to vaccination and challenge with salmon alphavirus (SAV) and Moritella viscosa

| PRJNA723861 | ENA

Significance of zinc on skin molecular signatures in Atlantic salmon with Winter ulcer disease

Project description:Vaccinated seawater adapted Atlantic salmon (average weight ~340 g) fed with variable levels of zinc were infected with Moritella viscosa at VESO Vikan. Early responses in skin to infection were investigated 4 days post challenge. Effect of chronic ulceration was investigated 35 days post challenge.

2021-04-09 | GSE171738 | GEO

Moritella viscosa

Project description:Moritella viscosa overview

| PRJNA1030455 | ENA

Responses to Moritella viscosa in skin and spleen of Atlantic salmon

Project description:Responses to Moritella viscosa in skin and spleen of Atlantic salmon

| PRJNA720609 | ENA

Co-cultivation and transcriptome sequencing of two co-existing fish pathogens Moritella viscosa and Aliivibrio wodanis.

Project description:Aliivibrio wodanis and Moritella viscosa have often been isolated together from fish with winter ulcer. Little is known about the interaction between the two bacterial species and how the presence of one bacterial species affects the behaviour of the other. The impact on bacterial growth in co-culture was investigated in vitro, and the presence of A. wodanis has a strong inhibitorial effect on M. viscosa. Further, we have sequenced the complete genomes of these two marine Gram-negative species, and have performed transcriptome analysis of the bacterial gene expression levels from in vivo samples. Using bacterial implants in the fish abdomen, we demonstrate that the presence of A. wodanis is altering the gene expression levels of M. viscosa compared to when the bacteria are implanted separately. The impeding effect on growth and the change in the global gene expression pattern of M. viscosa when the two pathogens co-exists is discussed in this paper.

2014-11-30 | E-MTAB-2851 | biostudies-arrayexpress

Moritella viscosa strain:J311

Project description:Moritella viscosa strain:J311 Raw sequence reads

| PRJNA1030280 | ENA

Global gene expression responses of Atlantic salmon skin to Moritella viscosa.

Project description:Moritella viscosa is a Gram-negative pathogen that causes large, chronic ulcers, known as winter-ulcer disease, in the skin of several fish species including Atlantic salmon. We used a bath challenge approach to profile the transcriptome responses of M. viscosa-infected Atlantic salmon skin at the lesion (Mv-At) and away from the lesion (Mv-Aw) sites. M. viscosa infection was confirmed through RNA-based qPCR assays. RNA-Seq identified 5212 and 2911 transcripts differentially expressed in the Mv-At compared to no-infection control and Mv-Aw groups, respectively. Also, there were 563 differentially expressed transcripts when comparing the Mv-Aw to control samples. Our results suggest that M. viscosa caused massive and strong, but largely infection site-focused, transcriptome dysregulations in Atlantic salmon skin, and its effects beyond the skin lesion site were comparably subtle. The M. viscosa-induced transcripts of Atlantic salmon were mainly involved in innate and adaptive immune response-related pathways, whereas the suppressed transcripts by this pathogen were largely connected to developmental and cellular processes. As validated by qPCR, M. viscosa dysregulated transcripts encoding receptors, signal transducers, transcription factors and immune effectors playing roles in TLR- and IFN-dependent pathways as well as immunoregulation, antigen presentation and T-cell development. This study broadened the current understanding of molecular pathways underlying M. viscosa-triggered responses of Atlantic salmon, and identified biomarkers that may assist to diagnose and combat this pathogen.

| S-EPMC8931016 | biostudies-literature

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