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RNA-seq analysis of HeLa cells in nutrient-rich and nutrient-deprived media

ABSTRACT: RNA-seq analysis of HeLa cells in nutrient-rich and nutrient-deprived media

PROVIDER: PRJNA868757 | ENA |

REPOSITORIES: ENA

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RNA-seq analysis of HeLa cells in nutrient-rich and nutrient-deprived media

Project description:RNA-seq analysis was applied in two independent batches, each one in triplicate samples of HeLa cells in nutrient-rich and nutrient-deprived media

2023-04-05 | GSE211066 | GEO

RNA-seq analysis of RIP of hnRNPQ and PABPN1 from HeLa cells in nutrient-deprived media

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RNA-seq analysis of RIP of hnRNPQ and PABPN1 from HeLa cells in nutrient-deprived media

Project description:RNA-seq analysis of RIP of hnRNPQ and PABPN1 from HeLa cells in nutrient-deprived media

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Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments

Project description:Biotransformation of soil organochlorine pesticides (OCP) is often impeded by a lack of nutrients relevant for bacterial growth and/or co-metabolic OCP biotransformation. By providing space-filling mycelia, fungi promote contaminant biodegradation by facilitating bacterial dispersal and the mobilization and release of nutrients in the mycosphere. We here tested whether mycelial nutrient transfer from nutrient-rich to nutrient-deprived areas facilitates bacterial OCP degradation in a nutrient-deficient habitat. The legacy pesticide hexachlorocyclohexane (HCH), a non-HCH-degrading fungus (Fusarium equiseti K3), and a co-metabolically HCH-degrading bacterium (Sphingobium sp. S8) isolated from the same HCH-contaminated soil were used in spatially structured model ecosystems. Using 13C-labelled fungal biomass and protein-based stable isotope probing (protein-SIP), we traced the incorporation of 13C fungal metabolites into bacterial proteins while simultaneously determining the biotransformation of the HCH isomers. The relative isotope abundance (RIA, 7.1 – 14.2%), labeling ratio (LR, 0.13 – 0.35), and the shape of isotopic mass distribution profiles of bacterial peptides indicated the transfer of 13C-labeled fungal metabolites into bacterial proteins. Distinct 13C incorporation into the haloalkane dehalogenase (linB) and 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (LinC), as key enzymes in metabolic HCH degradation, underpin the role of mycelial nutrient transport and fungal-bacterial interactions for co-metabolic bacterial HCH degradation in heterogeneous habitats. Nutrient uptake from mycelia increased HCH removal by twofold as compared to bacterial monocultures. Fungal-bacterial interactions hence may play an important role in the co-metabolic biotransformation of OCP or recalcitrant micropollutants (MPs).

2023-02-24 | PXD038571 | Pride

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