Project description:We have developed and optimized a reliable medium-throughput culture system for pig and human heart slices as a platform for testing the efficacy of novel heart failure therapeutics and reliable testing of cardiotoxicity in a 3D heart model.
Project description:Adult cardiac tissue undergoes a rapid process of dedifferentiation when cultured in vitro. The aim of this experiment was to compare the transcriptome of freshly prepared rat myocardial slices with slices cultured in unloaded conditions (no electromechanical stimulation) for 24 hours, electromechanical stimulation @ SL=1.8 for 24 hours & electromechanical stimulation @ SL=2.2 for 24 hours.
Project description:Transcriptional changes of mouse precision-cut liver slices (PCLS) after three days of culture were determined using RNA sequencing. PCLS were cultured for three days in the absence or presence of 2.5 mM valproic acid sodium salt (VPA). Illumina NovaSeq SP was used for sequencing.
Project description:Cultured organotypic cerebellar slices were exposed for different time points with either prions (RML) versus non-infectious brain homogenate (NBH) or ligands to the globular domain of the prion protein (POM1) vs IgG