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RNA sequencing of LSKs in the bone marrow from normal and 5-FU treated WT and Tespa1-null mice

ABSTRACT: RNA sequencing of LSKs in the bone marrow from normal and 5-FU treated WT and Tespa1-null mice

PROVIDER: PRJNA869724 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21066937_1.fastq.gz	Fastqsanger.gz
	SRR21066937_2.fastq.gz	Fastqsanger.gz
	SRR21066938_1.fastq.gz	Fastqsanger.gz
	SRR21066938_2.fastq.gz	Fastqsanger.gz
	SRR21066939_1.fastq.gz	Fastqsanger.gz

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RNA sequencing of LSKs in the bone marrow from normal and 5-FU treated WT and Tespa1-null mice

Project description:LSKs(lineage-, Sca-1+, c-kit+ cells) in the bone marrow from normal and 5-Fluorouracil (5-FU) treated (Day 9) wild type (WT) and Tespa1-knockout (KO) mice were isolated to explore the differentially expressed genes by RNA-seq.

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miRNA profiling data of mouse bone marrow samples four days post 5-FU treatment versus untreated controls

Project description:To identify miRNAs that regulate hematopoietic response after 5-FU-induced injury, we analyzed miRNA expression changes in bone marrow cells from mice with or without 5-FU treatment. Global miRNA expression profiling of the 5-FU and untreated cohorts showed that 92 miRNAs (among 168 that passed the detection threshold) were significantly upregulated or downregulated, with false discovery rates < 5%. Female 6wk-old C57Bl6/J mice were left untreated or given a single dose of 5-FU (150mg/kg). Four days post treatment whole bone marrow was isolated from each animal (n=3 for treated and n=5 for untreated controls) and lysed for total RNA using Trizol (Invitrogen). Samples were profiled for miRNA expression using a bead-based profiling methodology (Lu et al., 2008). Profiling data were normalized assuming equal total miRNA expression across samples. Differential miRNA expression was analyzed by studentM-bM-^@M-^Ys t-test and then calculated for false discovery rate using the Benjamini and Hochberg method.

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