Project description:The worldwide pest grape phylloxera (Daktulosphaira vitifoliae Fitch) is threatening vititulure. We investigated the compatible interaction with the host Teleki 5C (Vitis berlandiere Planch. X V. riparia Michx.) by investigating differential gene expression analysis using a custom made microarray. Samples (root tips and nodosities) were obtained from not infected and infected one-node cuttings four weeks after inoculation with 60 sibling phylloxera eggs. Four independent biological replicates each were analysed using a doul-color microarray experiment. Dye-swap hybridizations were performed. In total eight microarray were used for hybridization.
Project description:Herbivory plant-parasite interactions depend on the delivery of effector molecules by the invading insect species. Sedentary gall forming insects, such as grape phylloxera (Daktulosphaira vitifoliae FITCH, Phylloxeridae) secrete multiple effectors into host plant tissues that alter host cellular functions to the benefit of the insect. Analyses revealed 420 putative ‘DvEffectors’ were detected in salivary glands, dissected from root-feeding vs. starving D. vitifoliae larvae reared on Teleki 5C (V. berlandieri x V. riparia) under controlled growth conditions (25±3°C, 60% rH) by proteomic mass spectrometry and in-silico secretory prediction. Sixty-two conserved DvEffectors were shared with the aphid species A. pisum, M. persicae and R. padi including candidate effector proteins involved in feeding site establishment, plant defence suppression and nutrient uptake
