Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of depletion of METTL3 on gene expression of A549 cells

ABSTRACT: Effect of depletion of METTL3 on gene expression of A549 cells

PROVIDER: PRJNA870291 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Effect of depletion of METTL3 on gene expression of A549 cells

Project description:To investigate the function of METTL3 in lung cancer, we established shMETTL3 A549 cell line.

2024-03-03 | GSE211425 | GEO

METTL3 promotes translation in human cancer cells

Project description:we find METTL3 associates with polyribosomes and promotes translation. METTL3 depletion inhibits translation, and both wild-type and catalytically inactive METTL3 promote translation when tethered to the 3' untranslated region (UTR) of a reporter mRNA. Mechanistically, METTL3 enhances mRNA translation through an interaction with the translation initiation machinery. m6A seq in A549 and H1299 cells, RNA seq in METTL3 knockdown cells

2016-07-03 | E-GEOD-76367 | biostudies-arrayexpress

Effect of depletion of RALY on gene expression of A549 cells.

Project description:To investigate the effect of depletion of RALY on gene expression of A549 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq.

2023-05-24 | GSE209920 | GEO

Effect of depletion of RALY on gene expression of A549 cells.

Project description:Effect of depletion of RALY on gene expression of A549 cells.

| PRJNA862937 | ENA

Effect of SF3B4 depletion on gene expression of A549 lung cancer cell

Project description:Effect of SF3B4 depletion on gene expression of A549 lung cancer cell

| PRJNA922755 | ENA

Effect of depletion of ATG7 on gene expression in IAV-infected A549 cells

Project description:Effect of depletion of ATG7 on gene expression in IAV-infected A549 cells

| PRJNA869955 | ENA

Depletion of m6A methylation by conditional Mettl3 knockout in Ascl1-induced neurons

Project description:We have perturbed RNA methylation machinery and investigated the change in subcellular localization of mRNA in Ascl1-induced neurons (iNeurons). Mutant iNeuron line harbouring tagged endogenous Mettl3 was generated which allowed for the selective and targeted depletion of Mettl3 protein with the addition of dTAG. Cells were treated with dTAG for 40 hours then separated into compartments (neurites and soma) and then sequenced in parallel with samples which received no dTAG treatment.

2023-07-12 | E-MTAB-9643 | biostudies-arrayexpress

RNA-seq and Ribo-seq analysis of the effects of METTL3/14 depletion on gene expression during the interferon response in Huh7 cells

Project description:The RNA modification N6-methyladenosine (m6A) regulates gene expression through various transcript-specific effects. The overall goal of these experiments was to determine the effects of the m6A methyltransferase complex proteins METTL3 and METTL14 on the expression of interferon stimulated genes. First, we depleted METTL3 and METTL14 in Huh7 cells using siRNAs and treated with Mock or IFN-β to analyze the regulatory effects of METTL3/14 on interferon-stimulated genes' transcript abundance. We then used Ribo-seq following METTL3/14 siRNA depletion and IFN treatment to quantify its effect on the translation of ISGs and other genes. Overall, we found that METTL3/14 exerts transcript-specific regulatory effects on a subset of interferon-stimulated genes. Overall, METTL3/14 appeared to enhance the translation of a subset of interferon-stimulated genes. We characterized the molecular effects of METTL3/14 and m6A on certain ISGs and determined that m6A augments the antiviral effects of the interferon response.

2021-02-19 | GSE155447 | GEO

Effect of depletion of Elovl6 on gene expression

Project description:Effect of depletion of Elovl6 on gene expression

| PRJNA931962 | ENA

Effect of SF3B4 depletion on gene expression of A549 lung cancer cell

Project description:To investigate the function SF3B4 during the progression of lung cancer, A549 cells with either SF3B4 siRNA or scramble siRNA were used for RNA-seq.

2023-05-31 | GSE222598 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data