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Tobacco Smoke Exacerbates Filifactor alocis Pathogenicity

ABSTRACT: Tobacco Smoke Exacerbates Filifactor alocis Pathogenicity

PROVIDER: PRJNA870570 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21111153_1.fastq.gz	Fastqsanger.gz
	SRR21111153_2.fastq.gz	Fastqsanger.gz
	SRR21111154_1.fastq.gz	Fastqsanger.gz
	SRR21111154_2.fastq.gz	Fastqsanger.gz
	SRR21111155_1.fastq.gz	Fastqsanger.gz

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Tobacco Smoke Exacerbates Filifactor alocis Pathogenicity

Project description:RNA sequencing was used to identify genes up- and down-regulated when Fa was grown in cigarette smoke media

2023-03-08 | GSE211505 | GEO

Tobacco smoke exacerbates Filifactor alocis pathogenicity.

Project description:AimFilifactor alocis has recently emerged as a periodontal pathobiont that appears to thrive in the oral cavity of smokers. We hypothesized that identification of smoke-responsive F. alocis genes would provide insight into adaptive strategies and that cigarette smoke would enhance F. alocis pathogenesis in vivo.Materials and methodsF. alocis was grown in vitro and cigarette smoke extract-responsive genes determined by RNAseq. Mice were exposed, or not, to mainstream 1R6F research cigarette smoke and infected with F. alocis, or not, in an acute ligature model of periodontitis. Key clinical, infectious, and immune data were collected.ResultsIn culture, F. alocis growth was unaffected by smoke conditioning and only a small number of genes were specifically regulated by smoke exposure. Reduced murine mass, differences in F. alocis-cognizant antibody production, and altered immune profiles as well as altered alveolar bone loss were all attributable to smoke exposure and/or F. alocis infection in vivo.ConclusionsF. alocis is well-adapted to tobacco-rich conditions and its pathogenesis is enhanced by tobacco smoke exposure. A smoke-exposed ligature model of periodontitis shows promise as a tool with which to further unravel mechanisms underlying tobacco-enhanced, bacteria-induced disease.

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Transcriptome analysis of Filifactor alocis in co-culture with Porphyromonas gingivalis

Project description:We use high through put RNA sequenceing technology to study the genome-wide expression profile in an oral pathogen Filifactor alocis when co-cultured with another key-stone oral pathogen Porphyromonas gingivalis under anaerobic conditions and oxidative stress conditions.

2021-08-13 | GSE181879 | GEO

Proteomic analysis of Filifactor alocis type and clinically isolated strains

Project description:Filifactor alocis is a Gram-positive asaccharolytic, obligate anaerobic rod of the phylum Firmicutes, strongly associated with periodontitis. In this study, we performed the whole proteomic analysis of an F. alocis type strain (ATCC 35896; CCUG 47790), as well as nine clinically isolated strains at the Clinical Oral Microbiology Laboratory, Umeå University, for identification and deeper characterization of this species. Label-free quantification was used to compare the proteomic differences between different strains.

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Global changes in human neutrophil transcriptome profile during Filifactor alocis challenge

Project description:Periodontitis is a multifactorial chronic inflammatory infectious disease with a high prevalence in the adult population in the USA. Until recently, research into periodontitis focused primarily on putative pathogens such as Porphyromonas gingivalis. However, a reappraisal of microbial etiology has marked a turning point in the field due to the development of culture-independent techniques that allow the identification of bacterial species directly from nucleic acids. Filifactor alocis, a Gram positive anaerobic oral bacterium, has emerged as an important periodontal pathogen. Neutrophils are recruited in high numbers to the gingival tissue, where they are linked with protection against periodontal disease. However, periodontal pathogens evolved different strategies to resist neutrophil microbicidal mechanisms while propagating inflammation, which affords a source of nutrients. The goal of this study was to determine the global changes in human neutrophil gene expression induced by F. alocis challenge. RNA-sequencing analysis shows that F. alocis-challenge alters human neutrophil transcriptome including expression of genes associated with regulation of Toll-Like Receptor signaling pathway, MAPK signaling pathway, apoptosis, cytokines and chemokines, among other neutrophil effector functions.

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Project description:Oal anaerobic bacteria have to face constant oxidative stress in order to survive in the inflammatory environment of the periodontal pocket. In this study, we analyzed the transcriptome profiles of P. gingivalis and Filifactor in monoculture as compared to P. gingivalis+F.alocis coculture under H2O2 stress condtions, to analyze the genes responsible for enhanced survival of P. gingivalis in presence of F. alocis under oxidative-stress conditions.

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