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Single-nucleus RNA sequencing of OCTN2-defective engineered heart tissues and isogenic control

ABSTRACT: Single-nucleus RNA sequencing of OCTN2-defective engineered heart tissues and isogenic control

PROVIDER: PRJNA871255 | ENA |

REPOSITORIES: ENA

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Single-nucleus RNA sequencing of OCTN2-defective engineered heart tissues and isogenic control

Project description:Primary carnitine deficiency (PCD) is an autosomal recessive disorder caused by mutations in the gene SLC22A5, encoding for the plasmalemmal carnitine transporter OCTN2. PCD patients suffer from muscular weakness and dilated cardiomyopathy (DCM). However, currently available PCD models were unable to distinguish causative from secondary pathomechanisms. To further understand the contribution of cellular subclusters to the PCD disease phenotype we analyzed engineered heart tissues from OCTN2-defective genotype in comparison to isogenic control using single-nucleus RNA sequencing.

2022-08-22 | GSE211650 | GEO

RNA sequencing of control and dystrophin-mutant engineered heart tissues from human induced pluripotent stem cell-derived cardiomyocytes

Project description:We report the transcriptomic differences between engineered heart tissues generated from hiPSCs with a dystrophin-truncating mutation and an isogenic control. We find that the dystrophin mutation induces profound differences at the transcriptomic level and illuminates potential disease-causing mechanisms.

2022-08-31 | GSE199242 | GEO

Parthenogenetic stem cells for tissue-engineered heart repair

Project description:Uniparental parthenotes are considered an unwanted byproduct of in vitro fertilization. In utero parthenote development is severely compromised by defective organogenesis and in particular by defective cardiogenesis. Although developmentally compromised, apparently pluripotent stem cells can be derived from parthenogenetic blastocysts. Here we hypothesized that nonembryonic parthenogenetic stem cells (PSCs) can be directed toward the cardiac lineage and applied to tissue-engineered heart repair. We first confirmed similar fundamental properties in murine PSCs and embryonic stem cells (ESCs), despite notable differences in genetic (allelic variability) and epigenetic (differential imprinting) characteristics. Haploidentity of major histocompatibility complexes (MHCs) in PSCs is particularly attractive for allogeneic cell-based therapies. Accordingly, we confirmed acceptance of PSCs in MHC-matched allotransplantation. Cardiomyocyte derivation from PSCs and ESCs was equally effective. The use of cardiomyocyte-restricted GFP enabled cell sorting and documentation of advanced structural and functional maturation in vitro and in vivo. This included seamless electrical integration of PSC-derived cardiomyocytes into recipient myocardium. Finally, we enriched cardiomyocytes to facilitate engineering of force-generating myocardium and demonstrated the utility of this technique in enhancing regional myocardial function after myocardial infarction. Collectively, our data demonstrate pluripotency, with unrestricted cardiogenicity in PSCs, and introduce this unique cell type as an attractive source for tissue-engineered heart repair. 8 samples in total. Parthenogenetic stem cells (PSC): - PSC_1 - PSC_2 - PSC_3 Embryoid body (EB) assays of parthenogenetic stem cells: - EB_PSC_1 - EB_PSC_2 Embryonic stem cells (ESC): - ESC_1 - ESC_2 - ESC_3

2013-02-23 | E-GEOD-30868 | biostudies-arrayexpress

Heart Repair with Engineered Heart Muscle

Project description:Heart Repair with Engineered Heart Muscle

| PRJNA1154291 | ENA

Parthenogenetic stem cells for tissue-engineered heart repair

2013-02-23 | GSE30868 | GEO

Gene expression in engineered heart tissues exposed to THC and genistein

Project description:The goal of the experiment is to identify gene expression changes in engineered heart tissues (EHT) composed of human induced pluripotent stem cell-derived cardiomyocytes and endothelial cells treatered with Δ9-tetrahydrocannabinol (THC) or THC with genistein.

2022-03-19 | GSE198578 | GEO

Heart Repair with Engineered Heart Muscle

Project description:Releated to the single nucleus RNA sequencing data displayed in Extended Data Figure 1

2024-10-29 | GSE276021 | GEO

Gene expression in engineered heart tissues exposed to THC and genistein

Project description:Gene expression in engineered heart tissues exposed to THC and genistein

| PRJNA816073 | ENA

A CRISPR-engineered Isogenic Model Reveals Altered Neuronal Phenotypes of the 22q11.2 A-B Syndromic Deletion

Project description:A CRISPR-engineered Isogenic Model Reveals Altered Neuronal Phenotypes of the 22q11.2 A-B Syndromic Deletion

| PRJNA823698 | ENA

Differentially secreted proteins in KMT5B deficient-cells compared to their isogenic clone KMT5B wild-type assessed by mass spectrometry

Project description:In patient-derived DIPG models from multiple patients, with both H3K27M mutation and EZHIP overexpression, were generated CRISPR/Cas9-engineered and subclonal isogenic cells with KMT5B and/or KMT5C deficiency.

2021-07-20 | PXD026670 | Pride

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