Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expresssion analysis in liver and white adipose tissues from miR-322-503-351 knock out mice.

ABSTRACT: Gene expresssion analysis in liver and white adipose tissues from miR-322-503-351 knock out mice.

PROVIDER: PRJNA872003 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expresssion analysis in liver and white adipose tissues from miR-322-503-351 knock out mice.

Project description:To investigate the effect of miR-503 in aging associated type 2 diabetes, target genes of miR-503 need to be investigated. The global miR-322-503-351 deletion (KO) mouse was constructed, and RNA-seq was then performed on aged mouse liver and white adipose tissue (WAT).

2022-08-25 | GSE211749 | GEO

The effect of miR-322/-503 on myoblast differentiation in DM1 murine cell model

Project description:To study the gene expression profile difference caused by miR-322/-503 overexpression in the myoblast differentiation of DM1 group, we performed RNA-seq on the total RNA samples collected from the in vitro myoblast differentiation day 4 of control and miR-322/-503 overexpressing DM1 C2C12 cell models. The DM1 cell model was bulit by stably transfecting C2C12 cells with GFP-CUG200 plasmid. Each group contained three biological replicates. The expression matrix was obtained by Hisat2 followed by Stringtie.

2022-03-21 | GSE189897 | GEO

The effect of miR-322/-503 on myoblast differentiation in DM1 murine cell model

Project description:The effect of miR-322/-503 on myoblast differentiation in DM1 murine cell model

| PRJNA784964 | ENA

The miR-424(322)/503 gene cluster regulates pro- vs anti-inflammatory skin DC subset differentiation by modulating TGF-βsignaling

Project description:TGF-β family ligands are key regulators of dendritic cell (DC) differentiation and activation. Epidermal Langerhans cells (LCs) require TGF-β family signaling for their differentiation and canonical TGF-β1 signaling secures a non-activated LC state. LCs reportedly control skin inflammation and are replenished from peripheral blood monocytes, which also give rise to pro-inflammatory monocyte-derived DCs (moDCs). By studying mechanisms in inflammation, we previously screened LCs vs moDCs for differentially expressed miRNAs. miR-424/503 was the most strongly inversely regulated (moDCs > LCs). We found that miR-424/503 is induced during moDC differentiation and promotes moDC differentiation in human and mouse. Inversely, forced repression of miR-424 during moDC differentiation facilitated TGF-β1-dependent LC differentiation. Mechanistically, miR-424/503 deficiency in monocyte/DC precursors leads to the induction of TGF-β1-response genes critical for LC differentiation. Therefore, the miR-424/503 gene cluster plays a decisive role in anti-inflammatory LC vs pro-inflammatory moDC differentiation from monocytes.

2021-03-27 | GSE169717 | GEO

A microRNA cluster controls fat cell differentiation and adipose tissue expansion by regulating SNCG

Project description:The H19X-encoded miR-424(322)/503 cluster regulates multiple cellular functions. Here we report for the first time that it is also a critical linchpin of fat mass expansion. Deletion of this miRNA cluster in mice results in obesity, while increasing the pool of early adipocyte progenitors and hypertrophied adipocytes. Complementary loss and gain of function experiments and RNA sequencing demonstrate that miR-424(322)/503 regulates a conserved genetic program involved in the differentiation and commitment of white adipocytes. Mechanistically, we demonstrate that miR-424(322)/503 targets γ-Synuclein (SNCG), a factor that mediates this program rearrangement by controlling metabolic functions in fat cells, allowing adipocyte differentiation and adipose tissue enlargement. Accordingly, diminished miR-424(322) in mice and obese humans co-segregates with increased SNCG in fat and peripheral blood as mutually exclusive features of obesity, being normalized upon weight loss. Our data unveil a previously unknown regulatory mechanism of fat mass expansion tightly controlled by the miR-424(322)/503 through SNCG.

2021-10-13 | GSE185819 | GEO

Neurospora crassa 322

Project description:Neurospora crassa 322 genome sequencing

| PRJNA337492 | ENA

Sinorhizobium meliloti strain:322

Project description:Sinorhizobium meliloti 322 Resequencing

| PRJNA501526 | ENA

Bradyrhizobium japonicum strain:USDA 322 | isolate:USDA 322

Project description:Bradyrhizobium japonicum USDA 322 genome sequencing

| PRJNA1115780 | ENA

Streptomyces sp. WMMB 322

Project description:Streptomyces sp. WMMB 322 Genome sequencing