Project description:To investigate the effect of senescence induction on immunogical signatures in immunosuppresive B16F10 cells treated with the senescence inducer Doxorubicin
Project description:BMP2 treatment significantly reduces growth of B16F10 melanoma spheroids. This experiment analyses the transcriptome changes in B16F10 cells treated with or without BMP2.
Project description:Global gene expression profiling reveals a potential anti-melanoma effect of SQ-diEG in B16F10 cells. Squalene (SQ) was considered as a promising natural agent in anti-cancer treatment due to its strong antioxidant and anti-inflammatory activity; however, its pharmacological value has been largely underestimated because of its poor solubility and bioavailability. To adress this problem, a novel amphiphilic SQ derivative which bearing ethylene glycol oligomers was synthesized and was used as a permeation enhancer in this study to check its potential effect on anti-melanoma using B16F10 cells B16F10 were murine melanoma cells line, treated with 2.5 µM and 40 µM with all samples for 48 h. Microarray gene expression profiling was conducted for two biological replicates
Project description:We evaluated the changes in the transcriptome of spleen and bone marrow isolated myeloid cells in healthy and melanoma bearing animals and melanoma cell lines, B16F10 and YUMM3.3 using RNA-sequencing technologies.
Project description:To explore the effect of SIRPA perturbation on the expression changes of melanoma differentiation antigens (MDA), which directly mediates the immunogenicity of melanoma cells, we profiled the transcriptomes of B16F10 cells with SIRPA knockdown, overexpression, or control.