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Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I [leChRO-seq]

ABSTRACT: Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I [leChRO-seq]

PROVIDER: PRJNA874046 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21230600.fastq.gz	Fastqsanger.gz
	SRR21230601.fastq.gz	Fastqsanger.gz
	SRR21230602.fastq.gz	Fastqsanger.gz
	SRR21230603.fastq.gz	Fastqsanger.gz
	SRR21230604.fastq.gz	Fastqsanger.gz

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Similar Datasets

Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I [leChRO-seq]

Project description:During meiotic prophase I, germ cells must balance transcriptional activation with meiotic recombination and chromosome synapsis, biological processes requiring extensive changes to chromatin state and structure. Here we explored the interplay between chromatin accessibility and transcription across a detailed time-course of murine male meiosis by measuring genome-wide patterns of chromatin accessibility, transcription, and processed mRNA. To understand the relationship between these parameters of gene regulation and recombination, we integrated these data with maps of double-strand break formation. Maps of nascent transcription showed that Pol II is loaded on chromatin and maintained in a paused state early during meiosis. In pachynema, paused Pol II is released in a coordinated transcriptional burst resulting in ~3-fold increase in transcription. Release from pause is mediated by the transcription factor A-MYB and the testis-specific bromodomain protein, BRDT. The burst of transcriptional activity is both temporally and spatially segregated from key steps of meiotic recombination: double strand breaks show evidence of chromatin accessibility earlier during prophase I and at distinct loci from those undergoing transcriptional activation, despite shared chromatin marks. Our findings uncover the mechanisms of transcriptional activation and how cells compartmentalize transcription and recombination during meiosis.

2022-09-30 | GSE212118 | GEO

Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I [ATAC-Seq]

2022-09-30 | GSE212117 | GEO

Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I [RNA-Seq]

2022-09-30 | GSE212116 | GEO

Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I

Project description:Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I

| PRJNA874041 | ENA

Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I [RNA-Seq]

Project description:Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I [RNA-Seq]

| PRJNA874045 | ENA

Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I [ATAC-Seq]

Project description:Meiotic gene transcription programs are mediated by A-MYB and BRDT-dependent RNA Pol II pause release during mammalian prophase I [ATAC-Seq]

| PRJNA874047 | ENA

A-MYB and BRDT-dependent RNA Polymerase II pause release orchestrates transcriptional regulation in mammalian meiosis.

Project description:During meiotic prophase I, spermatocytes must balance transcriptional activation with homologous recombination and chromosome synapsis, biological processes requiring extensive changes to chromatin state. We explored the interplay between chromatin accessibility and transcription through prophase I of mammalian meiosis by measuring genome-wide patterns of chromatin accessibility, nascent transcription, and processed mRNA. We find that Pol II is loaded on chromatin and maintained in a paused state early during prophase I. In later stages, paused Pol II is released in a coordinated transcriptional burst mediated by the transcription factors A-MYB and BRDT, resulting in ~3-fold increase in transcription. Transcriptional activity is temporally and spatially segregated from key steps of meiotic recombination: double strand breaks show evidence of chromatin accessibility earlier during prophase I and at distinct loci from those undergoing transcriptional activation, despite shared chromatin marks. Our findings reveal mechanisms underlying chromatin specialization in either transcription or recombination in meiotic cells.

| S-EPMC10060231 | biostudies-literature

Genome-wide analysis of the distribution of the testis-specific double bromodomain protein BRDT reveals distinct roles in pachytene spermatocyte and round spermatids

Project description:BRDT, a member of the BET family of double bromodomain-containing proteins, is expressed uniquely in the testis from pachytene spermatocytes through round spermatids, and is essential for spermatogenesis in the mouse. Although BRDT is known to bind to acetylated lysines in chromatin, it is not known where in the genome BRDT binds or whether the sites vary during the complex stages of differentiation in which it is expressed. Herein, we use ChIP-Seq to identify genome-wide BRDT binding sites in chromatin from mouse male germ cells at two distinct stages of differentiation. We identified 5452 BRDT-bound genomic loci in pachytene spermatocytes and 5618 BRDT-bound genomic loci in round spermatids. Roughly two thirds of BRDT binding sites were located in genes and BRDT binding correlated with highly transcribed genes. BRDT binding sites also overlapped with several histone modifications or variants that are associated with active transcription. Motif analysis revealed that BRDT-bound regions are enriched for consensus sequences for the MYB, RFX, ETS and ELF1 in pachytene spermatocytes, and JunD, c-Jun, CRE and RFX in round spermatids. Taken together, our data suggest that BRDT regulates distinct downstream target genes and may have unique functions in the meiotic and spermiogenic transcription programs.

2021-01-26 | GSE98489 | GEO

Bromodomain-dependent stage-specific male genome programming by Brdt [Illumina BeadArray]

Project description:Male germ cell differentiation is a highly regulated multistep process initiated by the commitment of progenitor cells into meiosis and characterized by major chromatin reorganizations in haploid spermatids. We report here that a single member of the double bromodomain BET factors, Brdt, is a master regulator of both meiotic divisions and post-meiotic genome repackaging. Upon its activation at the onset of meiosis, Brdt drives and determines the developmental timing of a testis-specific gene expression program. In meiotic cells, Brdt initiates a genuine histone acetylation-guided programming of the genome by activating essential meiotic genes and repressing a “progenitor cells” gene expression program, while “priming” a post-meiotic gene group for further activation. At post-meiotic stages, a global chromatin hyperacetylation gives the signal for Brdt’s first bromodomain to direct the genome-wide replacement of histones by transition proteins. Brdt is therefore a unique and essential regulator of male germ cell differentiation, which, by using various domains in a developmentally controlled manner, first drives a specific spermatogenic gene expression program, and later controls the tight packaging of the male genome. Total RNA obtained from testes from prepuberal mice at 17dpp and at 20dpp were compared between Brdt-/- and Brdt+/- (17dpp), between Brdt-/- and Brdt +/+ (20dpp) and between BrdtBD1del and Brdt wt mice (20dpp). In each experiments, 6 replicates of each genotype and condition were used.

2012-08-30 | E-GEOD-39909 | biostudies-arrayexpress

Brdt KO mice: Wild-type vs. Mutant

Project description:Transcriptional profiling of mouse pituitary tissue comparing WT with Brdt KO mice. Goal was to determine the effects of Brdt absence on pituitary gene expression.

2016-03-18 | GSE72786 | GEO

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