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ABSTRACT: Evolution of extended-spectrum beta-lactamase-producing Escherichia coli

PROVIDER: PRJNA874473 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR21349900_1.fastq.gz	Fastqsanger.gz
	SRR21349900_2.fastq.gz	Fastqsanger.gz
	SRR21349901_1.fastq.gz	Fastqsanger.gz
	SRR21349901_2.fastq.gz	Fastqsanger.gz

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Project description:The emergence of colistin resistance in carbapenem-resistant and extended-spectrum ß-lactamase (ESBL)-producing bacteria is a significant threat to human health, and new treatment strategies are urgently required. Here we investigated the ability of the safe-for-human use ionophore PBT2 to restore antibiotic sensitivity in several polymyxin-resistant, ESBL-producing, carbapenem resistant Gram-negative human pathogens. PBT2 was observed to resensitize Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa to last-resort polymyxin class antibiotics, including a ‘next generation’ polymyxin derivative, FADDI-287. To gain additional insight into the potential mechanism of action of PBT2, we analyzed the transcriptome of K. pneumoniae and E. coli in the presence of sub-inhibitory concentrations of PBT2. Treatment with PBT2 was associated with multiple stress responses in both K. pneumoniae and E. coli. Significant changes in the transcription of transition metal ion homeostasis genes were observed in both strains.

2019-06-13 | GSE132637 | GEO

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