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Identify and characterize the polyploidization process of primary human proximal tubular cells (hPTC)

ABSTRACT: Identify and characterize the polyploidization process of primary human proximal tubular cells (hPTC)

PROVIDER: PRJNA874743 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21303700_1.fastq.gz	Fastqsanger.gz
	SRR21303700_2.fastq.gz	Fastqsanger.gz
	SRR21303701_1.fastq.gz	Fastqsanger.gz
	SRR21303701_2.fastq.gz	Fastqsanger.gz
	SRR21303702_1.fastq.gz	Fastqsanger.gz

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Identify and characterize the polyploidization process of primary human proximal tubular cells (hPTC)

Project description:We generated single-cell RNA sequencing (scRNAseq) datasets of primary cultures of human proximal tubular cells at different passages which we have found to contain a polyploid fraction. Within hPTC clusters, we found genes previously associated with different phases of endoreplication-mediated polyploidy and established that YAP1 controls the polyploidization process of hPTC. These observations were verified by treating hPTC with verteporfin (a YAP1 inhibitor), YAP1 silencing and by quantifying the expression of YAP1 downstream targets in sorted polyploid hPTC

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