Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

4sU-seq of MCMV

ABSTRACT: 4sU-seq of MCMV

PROVIDER: PRJNA874813 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21308114_1.fastq.gz	Fastqsanger.gz
	SRR21308114_2.fastq.gz	Fastqsanger.gz
	SRR21308115_1.fastq.gz	Fastqsanger.gz
	SRR21308115_2.fastq.gz	Fastqsanger.gz
	SRR21308116_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 104

Similar Datasets

4sU-seq of MCMV

Project description:Swiss murine embryonic fibroblasts (NIH-3T3) were infected with wild-type Smith strain mouse cytomegalovirus (MCMV) at a multiplicity of infection (MOI) of 10. Sequencing libraries were prepared using the 4sU-seq protocol (Dölken et al., RNA 2008 and Rutkowski et al.) from the indicated time points of infection as described in Rutkowski, A.J. et al, Nat. Commun (2015)

2023-04-26 | GSE212287 | GEO

dSLAM-seq of MCMV

Project description:Swiss murine embryonic fibroblasts (NIH-3T3) were infected with wild-type Smith strain mouse cytomegalovirus (MCMV) at a multiplicity of infection (MOI) of 10. Metabolic RNA labelling was performed using 400µM 4sU for one hour prior to cell lysis. Total RNA was isolated using the Trizol protocol and U-to-C conversions were induced by IAA treatment according to the SLAM-seq protocol (Herzog et al., Nature Methods 2017). Sequencing libraries were prepared using the dRNA-seq protocol from the indicated time points of infection as described by Whisnant A.W. et al, Nat. Commun (2020).

2023-04-26 | GSE212286 | GEO

cRNA-seq of MCMV

Project description:cRNA-seq of MCMV

| PRJNA874812 | ENA

dSLAM-seq of MCMV

Project description:dSLAM-seq of MCMV

| PRJNA874811 | ENA

Effect of 4sU labeling concentrations on quantification bias in nucleotide conversion RNA-seq data

Project description:To investigate the effect of increasing 4sU labeling concentrations on quantification bias in nucleotide conversion RNA-seq, we labeled 3 different cell lines with increasing concentrations of 4sU. We then measured 4sU dropout in 4sU samples compared to 4sU naive samples.

2024-02-06 | GSE229504 | GEO

Effect of 4sU labeling durations on quantification bias in nucleotide conversion RNA-seq data

Project description:To investigate the effect of labeling durations on quantification bias in nucleotide conversion RNA-seq, we labeled NIH cells with 4sU. We then measured 4sU dropout in 4sU samples compared to 4sU naive samples.

2024-02-06 | GSE229506 | GEO

cRNA-seq of MCMV

Project description:Swiss murine embryonic fibroblasts (NIH-3T3) were infected with wild-type Smith strain mouse cytomegalovirus (MCMV) at a multiplicity of infection (MOI) of 10. Sequencing libraries were prepared using the cRNA-seq protocol from the indicated time points of infection as described by Whisnant A.W. et al, Nat. Commun (2020)

2023-04-26 | GSE212285 | GEO

ATAC-seq, H3K9ac ChIP-seq and 4sU-seq in WT, SAGA and ATAC mutant mouse embryonic stem cells

Project description:This SuperSeries is composed of the ATAC-seq, H3K9ac ChIP-seq and 4sU-seq SubSeries.

2021-08-03 | GSE175905 | GEO

scRNA-Seq of negatively enriched NK cells 4 days post MCMV infection

Project description:Ly49G2+ NK cells mediate essential control of murine cytomegalovirus (MCMV) infection in mice which express the H-2Dk class I molecule. As a cognate ligand for specific Ly49G2 inhibitory receptor allotypes, H-2Dk also licenses Ly49G2+ NK cells in naïve and MCMV-infected mice. These findings suggest Ly49G2 may promote antiviral NK cell activities during MCMV infection. Indeed, in mice lacking the Ly49G2 receptor, MCMV resistance is fully abrogated. Additionally, NK cells expressing Ly49R, an NK cell associated activation receptor that also recognizes H-2Dk, have their function augmented by Ly49G2 and are required for MCMV resistance.

2019-12-11 | GSE132394 | GEO

Ribosome profiling of MCMV

Project description:Ribosome profiling of MCMV

| PRJNA874814 | ENA