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High resolution definition of the Staphylococcus aureus WalKR regulon [RNA-seq]

ABSTRACT: High resolution definition of the Staphylococcus aureus WalKR regulon [RNA-seq]

PROVIDER: PRJNA875041 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21315233.fastq.gz	Fastqsanger.gz
	SRR21315234.fastq.gz	Fastqsanger.gz
	SRR21315235.fastq.gz	Fastqsanger.gz
	SRR21315236.fastq.gz	Fastqsanger.gz
	SRR21315237.fastq.gz	Fastqsanger.gz

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High resolution definition of the Staphylococcus aureus WalKR regulon

Project description:High resolution definition of the Staphylococcus aureus WalKR regulon

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High resolution definition of the Staphylococcus aureus WalKR regulon [RNA-seq]

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Project description:The WalKR regulon was studied by comparing genes expression in a strain producing a constitutively active WalR regulator (WalRc) versus the wild type strain carrying the empty expression vector. Hybridizations were performed with three independent biological replicates. Our data allowed to identify 165 genes differentially expressed in the walRc expressing strain with a P-value ≤ 0.05 using Z-test and a threshold value of two-fold change in transcriptional levels. Among them, 108 genes are activated, and 57 genes are repressed. About 10% of these results were confirmed by quantitative real time PCR, revealing the high reliability of this overall study. Beyond the major effect of the WalKR system on genes expressing cell wall hydrolases, we have shown that it activates a large number of genes involved in virulence, and probably through its positive impact on the SaeRS two-component system, is a major activator of S. aureus virulence.

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Project description:Transcriptome analysis of the WalKR regulon in Staphylococcus aureus

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RNA-Seq-mediated transcriptome analysis of Staphylococcus aureus Newman wild-type, walKD119A, walKV149A and DHBP-treated wild-type strains

Project description:We reported the RNA-Seq results of Staphylococcus aureus Newman wild-type, walKD119A, walKV149A and DHBP-treated wild-type strains. We found that mutations of the potential signal transduction residues of WalK attenuate the activity of walKR two-component system, whereas DHBP supplementation activates this two-component system.

2016-01-15 | GSE75731 | GEO

High resolution definition of the Staphylococcus aureus WalKR regulon

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-09-01 | GSE212321 | GEO

RNA-seq reveals differential gene expression in Staphylococcus aureus at single-nucleotide resolution

Project description:Staphylococcus aureus is a gram-positive cocci and an important human commensal bacteria and pathogen. S. aureus infections are increasingly difficult to treat because of the emergence of highly resistant MRSA (Methicillin-resistant S. aureus) strains. Here we present a method to study differential gene expression in S. aureus using high-throughput RNA-sequencing (RNA-seq). We use RNA-seq to examine the differential gene expression in S. aureus RN4220 cells containing an exogenously expressed transcription factor and between two S. aureus strains (RN4220 and NCTC8325-4). The information provided by RNA-seq was a significant advance over previously described microarray based techniques. We investigated the sequence and gene expression differences between RN4220 and NCTC8325-4 and used the RNA-seq data to identify S. aureus promoters suitable for in vitro analysis. We used RNA-seq to describe, on a genome wide scale, genes positively and negatively regulated by a phage encoded transcription factor, gp67. RNA-seq offers the ability to study differential gene expression with single-nucleotide resolution, and is a considerable improvement over the predominant genome-wide transcriptome technologies used in S. aureus. RNA-seq analysis of Staphylococcus aureus RN4220 (electrocompetent strain) carrying either empty pRMC2 (inducible expression vector) or pRMC2 carrying the ORF67 gene (encodes gp67). Both strains were grown to OD 0.2 and transgene expression was induced with 100ng/ml anhydrotetracycline. As a control, Staphylococcus aureus strain NCTC8325-4 (non-electrocompetent strain) was grown under identical conditions except without the addition of anhydrotetracycline.

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