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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [RNA_seq]

ABSTRACT: The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [RNA_seq]

PROVIDER: PRJNA875131 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21355889_1.fastq.gz	Fastqsanger.gz
	SRR21355889_2.fastq.gz	Fastqsanger.gz
	SRR21355890_1.fastq.gz	Fastqsanger.gz
	SRR21355890_2.fastq.gz	Fastqsanger.gz
	SRR21355891_1.fastq.gz	Fastqsanger.gz

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [ncRNA_seq]

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [mRNA]

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities II

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [sRNA-seq]

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities [RNA_seq]

Project description:We profiled gene expression of AGO1 NTE truncation mutant lines to study the roles of the NTE region in Arabidopsis AGO1 involved RNA-induced silencing complex (RISC) formation.

2023-02-21 | GSE212362 | GEO

The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities.

Project description:microRNAs (miRNAs) regulate target gene expression through their ARGONAUTE (AGO) effector protein, mainly AGO1 in Arabidopsis thaliana. In addition to the highly conserved N, PAZ, MID and PIWI domains with known roles in RNA silencing, AGO1 contains a long, unstructured N-terminal extension (NTE) of little-known function. Here, we show that the NTE is indispensable for the functions of Arabidopsis AGO1, as a lack of the NTE leads to seedling lethality. Within the NTE, the region containing amino acids (a.a.) 91 to 189 is essential for rescuing an ago1 null mutant. Through global analyses of small RNAs, AGO1-associated small RNAs, and miRNA target gene expression, we show that the region containing a.a. 91-189 is required for the loading of miRNAs into AGO1. Moreover, we show that reduced nuclear partitioning of AGO1 did not affect its profiles of miRNA and ta-siRNA association. Furthermore, we show that the 1-to-90a.a. and 91-to-189a.a. regions of the NTE redundantly promote the activities of AGO1 in the biogenesis of trans-acting siRNAs. Together, we report novel roles of the NTE of Arabidopsis AGO1.

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Amyloids “at the border”: deep mutagenesis and random sequence extension reveal an incomplete amyloid-forming motif in Bri2 that turns amyloidogenic upon C-terminal extension

Project description:Stop-loss mutations cause over twenty different diseases. The effects of stop-loss mutations can have multiple consequences that are, however, hard to predict. Stop-loss in ITM2B/BRI2 results in C-terminal extension of the encoded protein and, upon furin cleavage, in the production of two 34 amino acid long peptides, ADan and ABri, that accumulate as amyloids in the brains of patients affected by familial Danish and British Dementia. To systematically explore the consequences of Bri2 C-terminal extension, here, we measure amyloid formation for 676 ADan substitutions and identify the region that forms the putative amyloid core of ADan fibrils, located between positions 20 and 26, where stop-loss occurs. Moreover, we measure amyloid formation for ~18,000 random C-terminal extensions of Bri2 and find that ~32% of these sequences can nucleate amyloids. We find that the amino acid composition of these nucleating sequences varies with peptide length and that short extensions of 2 specific amino acids (Aliphatics, Aromatics and Cysteines) are sufficient to generate novel amyloid cores. Overall, our results show that the C-terminus of Bri2 contains an incomplete amyloid motif that can turn amyloidogenic upon extension. C-terminal extension with de novo formation of amyloid motifs may thus be a widespread pathogenic mechanism resulting from stop-loss, highlighting the importance of determining the impact of these mutations for other sequences across the genome.

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The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-21 | GSE212365 | GEO

The N-terminal extension of Arabidopsis ARGONAUTE 1 is essential for microRNA activities II

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-21 | GSE224481 | GEO