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To identify candidate genes whose expressions were regulated by the histone H4 modifications during P. aeruginosa PA01 exposure

ABSTRACT: To identify candidate genes whose expressions were regulated by the histone H4 modifications during P. aeruginosa PA01 exposure

PROVIDER: PRJNA875662 | ENA |

REPOSITORIES: ENA

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To identify candidate genes whose expressions were regulated by the histone H4 modifications during P. aeruginosa PA01 exposure

Project description:Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for the histone modifications H4K5ac and H4K8ac in mid-L4 WT worms exposed to P. aeruginosa PA01 for 12 hours.

2022-09-04 | GSE212510 | GEO

Quantitative Analysis of mid-L4 WT worms after P. aeruginosa PA01 infection for 12 hours

Project description:To identify candidate genes whose expressions were regulated by P. aeruginosa PA01, we used RNA-Seq analysis to study the transcriptomic profiles of mid-L4 worms exposed to P. aeruginosa PA01 for 12 hours. Differential expression analysis was performed by comparing WT worms exposed to P. aeruginosa PA01 for 12 hours versus worms fed E. coli OP50.

2022-09-04 | GSE212511 | GEO

Identification of genes whose expressions are affected by hedgehog RNAi in spider embryos

Project description:In the spider Achaearanea tepidariorum, Hedgehog (Hh) signaling plays a key role in the formation of the two major embryonic axes. Analyses of expression patterns of the spider hh homolog, At-hh, suggested that Hh signaling might be involved in the subsequent segmentation process also. In this microarray experiment, we attempted to identify candidate genes whose expressions are regulated by Hh signaling during early phases of spider segmentation. The objective of this experiment was a screen for genes whose expressions might be affected by parental RNA interference (pRNAi) against At-hh in spider embryos. Oligonucleotide probes were designed based on accumulated A. tepidariorum EST sequences and embedded in custom microarrays (12K x2). Total RNA was extracted from late stage 5 embryos derived from a mated female before and after injection of dsRNA targeted for At-hh. Gene expression levels were compared between the untreated (normal) and the At-hh pRNAi-treated samples. Probes for At-hh and a homolog of Drosophila orthodenticle, At-otd, served as positive controls to validate this experiment.

2011-03-31 | E-GEOD-26468 | biostudies-arrayexpress

Azoarcus sp. PA01

Project description:Azoarcus sp. PA01 Genome sequencing and assembly

| PRJNA280039 | ENA

Mapping of H4-acetylated chromatin modifications in differentiating human primary myoblasts

Project description:We assessed the genomic distribution of H4-acetylated chromatin by ChIP using SNP chips and an antibody specific to four acetylated lysine residues of H4. Keywords: Comparative genomic hybridization

2006-08-28 | GSE4132 | GEO

Corynebacterium pseudotuberculosis strain:PA01

Project description:Corynebacterium pseudotuberculosis strain:PA01 Genome sequencing and assembly

| PRJNA304120 | ENA

Corynebacterium pseudotuberculosis strain:PA01

Project description:Corynebacterium pseudotuberculosis strain:PA01 Genome sequencing and assembly

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Rhizobium sp. H4 strain:H4

Project description:Rhizobium sp. H4 strain:H4 Genome sequencing and assembly

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CpBV-H4 ChIP-Seq

Project description:To address a target gene spectrum of CpBV-H4, we need to analyze total gene expression in response to a specific expression of CpBV-H4 by a transient expression technique.

2013-08-24 | E-MTAB-1835 | biostudies-arrayexpress

Exposure of cystic fibrosis bronchial epithelial cells (CFBE 41 o-) to Pseudomonas aeruginosa (PA01) biofilms

Project description:In the clinical setting, mutations in the CFTR gene enhance the inflammatory response to P. aeruginosa (PA01) infection, but measurements of the inflammatory response to pathogen stimulation by isolated airway epithelia can yield variable results. In this series, we exposed CFBE41o- cells over-expressing ?F508/?F508 CFTR and CFBE41o- cells rescued with wt-CFTR to P. aeruginosa biofilms. P. aeruginosa elicited a more robust increase in cytokine and chemokine expression (e.g., IL-8, CXCL2, CXCL3, CXCR4 and TNF-?) in CFBE-wt-CFTR cells compared to CFBE-?F508-CFTR cells. These results demonstrate that CFBE41o- cells complemented with wt-CFTR mount a more robust inflammatory response to P. aeruginosa than CFBE41o- ?F508/?F508-CFTR cells. CFBE41o- cells generously provided by Dr. J.P. Clancy (University of Alabama). The series involved 4 treatment groups: unexposed wild type CFTR cells, unexposed ?F508/?F508 CFTR cells, wild type CFTR cells exposed to PA01, and ?F508/?F508 CFTR cells exposed to PA01. Each treatment group involved 4 replicate polarized monolayers. PAO1 was added to the apical side of exposed monolayers at a multiplicity of infection (MOI) of 30:1 for 1 hour in the absence of antibiotics, and then planktonic PAO1 was removed by replacing the apical medium with MEM supplemented with 0.4% arginine (2). Control monolayers were treated identically except that vehicle only (medium used to grow PAO1, MEM supplemented with 0.4% arginine) was added to the apical side of cells. Five hours after washing planktonic P. aeruginosa from the cell monolayers mRNA was isolated.

2011-07-31 | E-GEOD-30439 | biostudies-arrayexpress

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