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Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells

ABSTRACT: Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells

PROVIDER: PRJNA877228 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21440800_1.fastq.gz	Fastqsanger.gz
	SRR21440800_2.fastq.gz	Fastqsanger.gz
	SRR21440801_1.fastq.gz	Fastqsanger.gz
	SRR21440801_2.fastq.gz	Fastqsanger.gz
	SRR21440802_1.fastq.gz	Fastqsanger.gz

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Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells

Project description:To determine the physiological roles of NCOA4 and ferritinophagy in macrophages, NCOA4 was depleted via liposome-mediated siRNA delivery into cells. Cells were treated with siRNA for NCOA4 depletion and then the transcriptome profiles acquired from RNA-seq were compared with those of control (scramble siRNA-treated) cells.

2022-09-30 | GSE212772 | GEO

Effect of iron depletion on gene expression of NCOA4-deficient and control HT22 hippocampal neuron cells

Project description:To determine the physiological roles of NCOA4 and NCOA4-mediated ferritinophagy in hippocampal neurons, NCOA4 was depleted using siRNA and desferrioxamine (DFO; 100 µM, 24 h) was added to restrict iron availability. Cells were treated for NCOA4 depletion, iron restriction, or both, and then the transcriptome profiles acquired from RNA-seq were compared with those of control (scramble siRNA-treated) cells.

2022-12-30 | GSE211931 | GEO

The effect of talc on J774 cells

Project description:The effect of talc on J774 cells

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Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis

Project description:Macrophages play an essential role in the early immune response to Mycobacterium tuberculosis and are the cell type preferentially infected in vivo. Primary macrophages and macrophage-like cell lines are commonly used as infection models, although the physiological relevance of cell lines, particularly for host-pathogen interaction studies, is debatable. Here we use high-throughput RNA-sequencing to analyse transcriptome dynamics of two macrophage models in response to M. tuberculosis infection. Specifically, we study the early response of bone marrow-derived mouse macrophages and cell line J774 to infection with live and -irradiated (killed) M. tuberculosis. We show that infection with live bacilli specifically alters the expression of host genes such as RSAD2, IFIT1/2/3 and RIG-I, whose potential roles in resistance to M. tuberculosis infection have not yet been investigated. In addition, the response of primary macrophages is faster and more intense than that of J774 cells in terms of number of differentially expressed genes and magnitude of induction/repression. Our results point to potentially novel processes leading to immune containment early during M. tuberculosis infection, and support the idea that important differences exist between primary macrophages and cell lines, which should be taken into account when choosing a macrophage model to study host-pathogen interactions.

2016-10-18 | GSE88801 | GEO

The effect of macrophage depletion on mammary basal cells

Project description:The effect of macrophage depletion on mammary basal cells

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The effect of macrophage depletion on mammary basal cells

Project description:To investigate the effect of macrophages on mammary basal cells, we established a macrophage depletion mouse model by clodronate liposome (CL) intraperitoneal injection, and the mammary basal cells were collected by FACS sorting. We then performed RNA-seq analysis on clodronate liposome (CL) treated mammary basal cells compared to control.

2023-04-20 | GSE210268 | GEO

Macrophage depletion blocks congenital SARM1-dependent neuropathy

Project description:Macrophage depletion blocks congenital SARM1-dependent neuropathy

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addition of proteasome inhibitor to J774 cells

Project description:J774 cells were treated with MG132, epoxomicin and ONX-0912 for 4 hours, and gene expression was evaluated In order to evaluate the molecular mechanism of inflammatory responses in PRAAS(Proteasome-Associated Autoinflammatory Syndromes ), we established Psmb8-KI mouse that harbors the same mutation in human patients. Psmb8-KI mice showed higher susceptibility to imiquimod-induced skin inflammation (IMS). DNA microarray analysis showed that treatment of J774 cells with proteasome inhibitors increased the expression of the Cxcl9 and Cxcl10 genes, and the skins where imiquimod was painted also expressed both genes at higher levels in Psmb8-KI than control mice. Deficiency in Cxcr3, the gene encoding the receptor of CXCL9 and CXCL10, in wild-type mice did not change IMS susceptibility, while deficiency in Cxcr3 in Psmb8-KI mice ameliorated IMS.

2022-04-01 | GSE189308 | GEO

C1q elicited macrophage gene expression

Project description:We have data from a previous microarray experiment which shows that C1q elicits macrophages to express genes important for clearance of dead (apoptotic) cells. In this experiment we want to determine the signaling mechanism by which C1q is turning on macrophage gene expression. For our control (sample 1-3) we heated C1q to denature it so it is inactive. We also want to know which portion of C1q is responsible for activation the macrophage so we generated C1qtails (samples 4-5). We also have macrophages treated with the whole C1q molecule (7-9). And finally we have a cell line (J774) treated with C1q but does not activate the cells (samples 10-12). Bone marrow derived macrophages or J774 cells were adhered to heat inactivated C1q, C1q tails or C1q for 18 hours. Cells were harvested and RNA was isolated and converted to double stranded cDNA and subsequently labeled and hybridized onto NimbleGen mouse gene expression array [100718_MM9_EXP] using Nimblegen Hybridization system 4 according to manufacturerM-bM-^@M-^Ys instructions (Roche)

2014-07-10 | E-GEOD-48619 | biostudies-arrayexpress

J774 Macrophage proteins interacion with Paracoccidioides lutzii GAPDH

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2019-07-29 | PXD008309 | Pride

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