Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of circular RNAs, long non-coding RNAs ,and mRNAs in human keloid dermal tissues

ABSTRACT: RNA sequencing of circular RNAs, long non-coding RNAs ,and mRNAs in human keloid dermal tissues

PROVIDER: PRJNA878513 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA sequencing of circular RNAs, long non-coding RNAs ,and mRNAs in human keloid dermal tissues

Project description:Keloid is a dermal fibroproliferative disease with various etiologies and unclear pathogenesis. Recent studies have revealed that circular RNAs (circRNAs) and long non-coding RNAs (lncRNAs) exerted regulatory functions through a competing endogenous RNA (ceRNA) pathway in keloid progression. However, the expression profiles of circRNAs and lncRNAs in keloid dermal tissues (KDTs) remain unknown. This study aimed to identify differentially expressed circRNAs, lncRNAs and genes in KDTs.

2023-09-07 | GSE212954 | GEO

A microarray of circular RNAs in human keloid dermal fibroblasts

Project description:To further explore the expression of circular RNAs in keloid，we have completed the Arraystar Human circRNA Array V2 analysis of the 8 samples，including 4 patients-derived keloid dermal fibroblasts and 4 normal dermal fibroblasts.

2022-02-23 | GSE184097 | GEO

A microarray of circular RNAs in human keloid dermal fibroblasts

Project description:A microarray of circular RNAs in human keloid dermal fibroblasts

| PRJNA763180 | ENA

Effects of ECM rigidity on normal and keloid dermal fibroblasts

Project description:This study investigated the effects of extracellular matrix (ECM) rigidty on gene expression patterns in normal dermal fibroblasts (NDFs) and keloid dermal fibroblasts (KDFs). Cells were cultured on collagen coated polyacrylamide hydrogels with elastic moduli mimicking normal skin (8 kPa) or keloid scar tissue (214 kPa), and changes in gene expression were profiled using next-generation RNA-sequencing. Differential gene expression analysis identified overall significant differences in gene expression between the NDF and KDF populations. Despite high levels of inter-patient heterogeneity in the KDF samples, further principal components analysis revealed a subset of genes (PC5) that were specifically regulated by ECM rigidity. Gene set enrichment analysis of the PC5 genes identified classic pathways associated with mechanotransduction, including Hippo Signalling and Regulation of the Actin Cytoskeleton, as well as genes associated with the Autophagy pathway. Additional in vitro studies and human tissue staining confirmed the biomechanical regulation of autophagic flux in NDFs and KDFs and differential remodelling of the lysosome in KDFs and keloid scars. Together, these findings implicate autophagy and lysosomal remodelling as biomechanically dysregulated pathways in keloid fibroblasts, and these mechanisms may contribute to scar pathogenesis.

2023-11-02 | GSE246562 | GEO

Profiling of mRNAs, long chain non-coding RNAs and circular RNAs in gastric cancer tissues and adjacent normal tissues

Project description:Purpose: To investigate the role and mechanism of mRNAs, long chain non-coding RNAs and circular RNAs in gastric cancer. Methods: RNA-seq of ribosomal RNA-depleted total RNA were performed to screen differential expressed mRNAs, long chain non-coding RNAs between paired gastric cancer tissues and adjacent normal tissues.For the linear RNA was digested with 3 U of RNase R per µg of RNA. Results: A total of 83672 mRNAs, 105998 long chain non-coding RNAs, 25441 distinct circRNAs were identified in these samples, and 13929 of these circRNAs were identified as novel circRNAs.

2022-03-15 | GSE193453 | GEO

Circular DNA tumor viruses make circular RNAs

Project description:Circular DNA tumor viruses make circular RNAs

| PRJNA483204 | ENA

Identification of circular RNAs and mRNAs expression profiles in microglia M2 polarization

Project description:Identification of circular RNAs and mRNAs expression profiles in microglia M2 polarization

| PRJNA819876 | ENA

Identification and characterization of MCF01A circular RNAs [RNA-seq]

Project description:Circular RNAs (circRNAs) are widespread circular forms of non-coding RNAs with largely unknown function. Because stimulation of mammary cells with the epidermal growth factor (EGF) leads to dynamic changes in the abundance of both coding and non-coding RNA molecules, and culminates in the acquisition of a robust migratory phenotype, this cellular model might disclose functions of circRNAs. Here we show that circRNAs of EGF-stimulated mammary cells are stably expressed, while mRNAs and micro-RNAs change within minutes. In general, the circRNAs we detected are relatively long-lived and weakly expressed. Interestingly, they are almost ubiquitously co-expressed with the corresponding linear transcripts, and the respective, shared promoter regions are more active compared to genes producing linear isoforms only. These findings imply that altered abundance of circRNAs, unlike changes in the levels of other RNAs, might not play critical roles in signaling cascades and downstream transcriptional networks that rapidly commit cells to specific outcomes. Detection of circRNAs from RNA-Seq â triplicate

2015-12-17 | E-GEOD-71195 | biostudies-arrayexpress

Identification and characterization of MCF01A circular RNAs [ChIP-seq]

2015-12-17 | E-GEOD-71196 | biostudies-arrayexpress

Identification of circular RNAs and mRNAs expression profiles in microglia M2 polarization [CircRNA]

Project description:Identification of circular RNAs and mRNAs expression profiles in microglia M2 polarization [CircRNA]

| PRJNA819881 | ENA