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Engineered cell entry links receptor biology with single-cell genomics [bulk RNA-Seq]

ABSTRACT: Engineered cell entry links receptor biology with single-cell genomics [bulk RNA-Seq]

PROVIDER: PRJNA878678 | ENA |

REPOSITORIES: ENA

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Engineered cell entry links receptor biology with single-cell genomics

Project description:Engineered cell entry links receptor biology with single-cell genomics

| PRJNA878699 | ENA

Engineered cell entry links receptor biology with single-cell genomics [scRNA-Seq]

Project description:Engineered cell entry links receptor biology with single-cell genomics [scRNA-Seq]

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Engineered cell entry links receptor biology with single-cell genomics.

Project description:Cells communicate with each other via receptor-ligand interactions. Here, we describe lentiviral-mediated cell entry by engineered receptor-ligand interaction (ENTER) to display ligand proteins, deliver payloads, and record receptor specificity. We optimize ENTER to decode interactions between T cell receptor (TCR)-MHC peptides, antibody-antigen, and other receptor-ligand pairs. A viral presentation strategy allows ENTER to capture interactions between B cell receptor and any antigen. We engineer ENTER to deliver genetic payloads to antigen-specific T or B cells to selectively modulate cellular behavior in mixed populations. Single-cell readout of ENTER by RNA sequencing (ENTER-seq) enables multiplexed enumeration of antigen specificities, TCR clonality, cell type, and states of individual T cells. ENTER-seq of CMV-seropositive patient blood samples reveals the viral epitopes that drive effector memory T cell differentiation and inter-clonal vs. intra-clonal phenotypic diversity targeting the same epitope. ENTER technology enables systematic discovery of receptor specificity, linkage to cell fates, and antigen-specific cargo delivery.

| S-EPMC9789208 | biostudies-literature

Engineered cell entry links receptor biology with single-cell genomics [scRNA-Seq]

Project description:Cells communicate with each other via receptor-ligand interactions. Here we describe lentiviral-mediated cell e¬ntry by engineered receptor-ligand interaction (ENTER) to display ligand proteins, deliver payloads, and record receptor specificity. We optimize ENTER to decode interactions between T cell receptor (TCR)-MHC peptides, antibody-antigen, and other receptor-ligand pairs. A viral presentation strategy allows ENTER to capture interactions between B cell receptor and any antigen. We engineer ENTER to deliver genetic payloads to antigen-specific T or B cells to selectively modulate cellular behavior in mixed populations. Single-cell readout of ENTER by RNA-sequencing (ENTER-seq) enables multiplexed enumeration of antigen specificities, TCR clonality, cell-type and states of individual T cells. ENTER-seq of CMV-seropositive patient blood samples reveals the viral epitopes that drive effector memory T cell differentiation and inter- vs intra-clonal phenotypic diversity targeting the same epitope. ENTER technology enables systematic discovery of receptor specificity, linkage to cell fates, and antigen-specific cargo delivery.

2022-12-12 | GSE212996 | GEO

Engineered cell entry links receptor biology with single-cell genomics [bulk RNA-Seq]

2022-12-12 | GSE212984 | GEO

Engineered cell entry links receptor biology with single-cell genomics

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-12-12 | GSE212998 | GEO

Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology

Project description:Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology

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Dissecting engineered cell types and enhancing cell fate conversion via CellNet

Project description:Engineering clinically relevant cells in vitro holds promise for regenerative medicine, but most protocols fail to faithfully recapitulate target cell properties. To address this, we developed CellNet, a network biology platform that determines whether engineered cells are equivalent to their target tissues, diagnoses aberrant gene regulatory networks, and prioritizes candidate transcriptional regulators to enhance engineered conversions. Using CellNet, we improved B cell to macrophage conversion, transcriptionally and functionally, by knocking down predicted B cell regulators. Analyzing conversion of fibroblasts to induced hepatocytes (iHeps), CellNet revealed an unexpected intestinal program regulated by the master regulator Cdx2. We observed functional engraftment of mouse colon by iHeps, thereby establishing their broader potential as endoderm progenitors and demonstrating direct conversion of fibroblasts into intestinal epithelium. Our studies illustrate how CellNet can be employed to improve direct conversion and to uncover unappreciated properties of engineered cells. 15 samples

2014-08-14 | E-GEOD-59037 | biostudies-arrayexpress

YAP promotes cardiomyocyte cell cycle entry

Project description:YAP promotes cardiomyocyte cell cycle entry

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MILK-Omics: Systems Biology of Human Milk and its Links to Maternal and Infant Health

Project description:MILK-Omics: Systems Biology of Human Milk and its Links to Maternal and Infant Health

| PRJNA1010893 | ENA