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Cleavage Under Targets and Tagmentation analysis of human lung lymphatic endothelial cells

ABSTRACT: Cleavage Under Targets and Tagmentation analysis of human lung lymphatic endothelial cells

PROVIDER: PRJNA878816 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21504638_1.fastq.gz	Fastqsanger.gz
	SRR21504638_2.fastq.gz	Fastqsanger.gz
	SRR21504639_1.fastq.gz	Fastqsanger.gz
	SRR21504639_2.fastq.gz	Fastqsanger.gz
	SRR21504640_1.fastq.gz	Fastqsanger.gz

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Cleavage Under Targets and Tagmentation analysis of human lung lymphatic endothelial cells

Project description:Fluid clearance mediated by lymphatic vessels is known to be essential for lung inflation and gas exchange function during the transition from prenatal to postnatal life, yet the molecular mechanisms that regulate lymphatic function remain unclear. Here, we profiled the molecular features of lymphatic endothelial cells (LECs) in embryonic and postnatal day (P) 0 lungs by single-cell RNA-seq analysis. We identified that the expression of c-JUN is transiently upregulated in P0 LECs. Conditional knockout of Jun in LECs impairs the opening of lung lymphatic vessels at birth, leading to fluid retention in the lungs and neonatal death. We further demonstrated that increased mechanical pressure induces the expression of c-JUN in LECs. c-JUN regulates the opening of lymphatic vessels by modulating remodeling of the actin cytoskeleton in LECs. Our study established the essential regulatory function of c-JUN-mediated transcriptional responses in facilitating lung lymphatic fluid clearance at birth.

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