Project description:Transcriptional profiles of mid-exponentially growing Salmonella enterica sv Enteritidis PT8 cultures in response to exposure to trans-cinnamaldehyde (0.01%; 0.75mM) or eugenol (0.04%; 2.46mM)
Project description:Cinnamaldehyde is a natural antimicrobial and has been found to be effective against many foodborne pathogens including Escherichia coli O157:H7. Although its antimicrobial effects have been well investigated, limited information is available on its effects at the molecular level. Sublethal treatment at 200 mg/l cinnamaldehyde inhibited growth of E. coli O157:H7 at 37oC and for M-bM-^IM-$ 2 h caused cell elongation, but from 2 to 4 h growth resumed and cells reverted to normal length. To understand this transient behaviour, genome-wide transcriptional analysis of E. coli O157:H7 was performed at 2 and 4 h exposure to cinnamaldehyde. Drastically different gene expression profiles were obtained at 2 and 4 h. At 2 h exposure, cinnamaldehyde induced overexpression of many oxidative stress-related genes, reduced DNA replication, and synthesis of protein, O-antigen and fimbriae. At 4 h, many cinnamaldehyde-induced repressive effects on E. coli O157:H7 gene expressions were reversed and oxidatve stress genes were nolonger differentially expressed. Duplicate E. coli O157:H7 cultures with or without 200 mg/l cinnamaldehyde were incubated at 37M-BM-0C for M-bM-^IM-$ 4 h. Cinnamaldehyde-induced changes in gene expression profiles were compared at 2 and 4 h using Affymetrix Ginechip 2.0 microarrays.
Project description:Cinnamaldehyde is a natural antimicrobial and has been found to be effective against many foodborne pathogens including Escherichia coli O157:H7. Although its antimicrobial effects have been well investigated, limited information is available on its effects at the molecular level. Sublethal treatment at 200 mg/l cinnamaldehyde inhibited growth of E. coli O157:H7 at 37oC and for ≤ 2 h caused cell elongation, but from 2 to 4 h growth resumed and cells reverted to normal length. To understand this transient behaviour, genome-wide transcriptional analysis of E. coli O157:H7 was performed at 2 and 4 h exposure to cinnamaldehyde. Drastically different gene expression profiles were obtained at 2 and 4 h. At 2 h exposure, cinnamaldehyde induced overexpression of many oxidative stress-related genes, reduced DNA replication, and synthesis of protein, O-antigen and fimbriae. At 4 h, many cinnamaldehyde-induced repressive effects on E. coli O157:H7 gene expressions were reversed and oxidatve stress genes were nolonger differentially expressed.
Project description:Through RNA sequencing, we report here that Carvacrol (CAR) affected the expression of 153 genes which included the down-regulation of energy generation-related proteins, pentose phosphate pathway, and up-regulation of ribosomes and translation-related proteins. Few differentially expressed genes were found when M. bovis were treated with Trans-cinnamaldehyde (TC), Eugenol (EU), or Carvacrol (CAR) when the three PDAs were double or triple combined. Our results suggest that, as opposed to the effect of CAR, the growth inhibitory effects of TC and EU at levels tested may be exerted through mechanisms other than gene expression regulations.
Project description:Gene expression response of Salmonella enterica serovar Enteritidis phage type 8 to subinhibitory concentrations of trans-cinnamaldehyde and eugenol
| PRJNA397848 | ENA
Project description:cinnamaldehyde supplementation from Chicken in LPDs
| PRJNA1176335 | ENA
Project description:Gut microbiome of rats treated with Cinnamaldehyde
