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Echinacea purpurea

ABSTRACT: RNA seq of Echinacea purpurea under MeJA treatment

PROVIDER: PRJNA879270 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21594369_1.fastq.gz	Fastqsanger.gz
	SRR21594369_2.fastq.gz	Fastqsanger.gz
	SRR21594370_1.fastq.gz	Fastqsanger.gz
	SRR21594370_2.fastq.gz	Fastqsanger.gz
	SRR21594371_1.fastq.gz	Fastqsanger.gz

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Project description:To seek scientific evidence supporting therapeutic use of Echinacea, the transcriptomic effects of Echinacea purpurea extract on mouse bone marrow-derived dendritic cells (BMDCs) were analyzed using primary cultures. Affymetrix DNA microarray and bioinformatics analyses of genes differentially expressed in DCs treated with [BF/S+L/Ep] for 4 or 12 h revealed that the majority of responsive genes were related to cell adhesion or motility (Cdh10, Itga6, Cdh1, Gja1 and Mmp8), or were chemokines (Cxcl2, Cxcl7) or signaling molecules (Nrxn1, Pkce and Acss1).

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Transcription profiling of human dendritic cells treated with Echinacea purpurea phytocompounds

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Subtracted Diversity Array identifies novel molecular markers including retrotransposons for fingerprinting Echinacea species

Project description:Echinacea, native to the Canadian prairies and the prairie states of the United States, has a long tradition as a folk medicine for the Native Americans. Currently, Echinacea are among the top 10 selling herbal medicines in the U.S. and Europe, due to increasing popularity for the treatment of common cold and ability to stimulate the immune system. However, the genetic relationships within the species of this genus are unclear, which makes difficult the authentication of the species used for the medicinal industry. We report the construction of a novel Subtracted Diversity Array (SDA) for Echinacea species and demonstrate the potential of this array for isolating highly polymorphic sequences. In order to selectively isolate Echinacea-specific sequences, a Suppression Subtractive Hybridization (SSH) was performed between a pool of twenty-four Echinacea genotypes and a pool of other angiosperms and non-angiosperms. A total of 283 subtracted genomic DNA (gDNA) fragments were amplified and arrayed. Twenty-seven Echinacea genotypes, including four that were not used in the array construction, could be successfully discriminated. Interestingly, unknown samples of E. paradoxa and E. purpurea could be unambiguously identified from the cluster analysis. Furthermore, this Echinacea-specific SDA was also able to isolate highly polymorphic sequences. Five out of the eleven most discriminatory features matched to known retrotransposons.

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Project description:Echinacea purpurea hairy root treatment with MeJA

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