Project description:The goal of the study was to identify the gene changes that occur in the striatum of neonatal piglets exposed to cardiopulmonary bypass (CPB) with deep hypothermic cardiac arrest (DHCA) then recovered for 6h. To accomplish our goal, mRNA-seq was performed on RNA isolated from neonatal pigs exposed to either DHCA or sham conditions.
Project description:Transcriptional profiling of miRNAs from rat brain tissues comparing controls (Sham) with ischemic rats (tMCAO) and neuroprotected rats (RLIP) Internal normalization: ischemic core vs. periischemic and ANOVA comparison across three experimental conditions: Sham, tMCAO and RLIP
Project description:Samples from three groups, SHAM operated group and two STROKE operated groups treated two days post operation with AAV1-eGFP control and AAV1-MANF treatment were sequenced and analysed for differential gene expression
Project description:To determine the changes in intra-renal gene expression in a novel large animal model of post Cardiopulmonary Bypass (CPB) acute kidney injury, we collected renal medulla samples obtained 24hours post intervention. The transcriptional profile of the mRNA in these samples was measured with gene array technology. Pigs were subjected to 2.5 hours of general anaesthesia or 2.5 hours of CPB under general anaesthesia. Renal medulla samples were collected 24 hours post intervention.
