Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expanding the prostate cancer cell line repository: insights into the first Caribbean cell line, ACRJ-PC28

ABSTRACT: Expanding the prostate cancer cell line repository: insights into the first Caribbean cell line, ACRJ-PC28

PROVIDER: PRJNA879775 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Expanding the prostate cancer cell line repository: insights into the first Caribbean cell line, ACRJ-PC28

Project description:Prostate cell lines from diverse backgrounds are important to addressing disparities in prostate cancer (PCa) incidence and mortality rates among Black men. ACRJ-PC28 was developed from a transrectal needle biopsy and established via inactivation of the CDKN2A locus and simultaneous expression of human telomerase. Characterization assays included growth curve analysis, immunoblots, IHC, 3D cultures, immunofluorescence imaging, confocal microscopy, flow cytometry, WGS and, RNA-Seq. RNA-Seq confirmed the expression of prostate specific genes alpha-methylacyl-CoA racemase (AMACR) and NKX3.1 and Neuroendocrine specific markers, synaptophysin (SYP) and enolase 2 (ENO2) and IHC confirmed the presence of AMACR. WGS confirms the absence of exonic mutations and the presence of intronic variants that appear to not affect function of AR, p53 and pRB. RNA-Seq data revealed numerous TP53 and RB1 mRNA splice variants and the lack of AR mRNA expression. The novel cell line described here should advance research addressing the disparity in prostate cancer among Black men.

2022-09-15 | GSE213223 | GEO

Microarray studies of darkness stress and bleaching in the Caribbean corals Acropora palmata and Montastraea

Project description:This SuperSeries is composed of the following subset Series: GSE27022: Microarray studies of darkness stress and bleaching in the Caribbean coral Acropora palmata GSE27024: Microarray studies of darkness stress and bleaching in the Caribbean coral Montastraea faveolata Refer to individual Series

2011-03-22 | E-GEOD-27025 | biostudies-arrayexpress

The National Institute of Neurological Disorders and Stroke (NINDS) Human Genetics Resource Center: DNA and Cell Line Repository (the NINDS Repository): Cerebrovascular Disease/Stroke Study

Project description:The National Institute of Neurological Disorders and Stroke (NINDS) Human Genetics Resource Center: DNA and Cell Line Repository (the NINDS Repository): Cerebrovascular Disease/Stroke Study

| PRJNA75385 | ENA

Merkel cell carcinoma Tissue and Data Repository

Project description:Merkel cell carcinoma Tissue and Data Repository

| PRJNA671169 | ENA

NIGMS Human Genetic Cell Repository: Ethnic Panels

Project description:NIGMS Human Genetic Cell Repository: Ethnic Panels

| PRJNA75409 | ENA

Merkel cell carcinoma Tissue and Data Repository

Project description:Merkel cell carcinoma Tissue and Data Repository

| PRJNA671168 | ENA

NIGMS Human Genetic Cell Repository: CEPH Family Panels

Project description:NIGMS Human Genetic Cell Repository: CEPH Family Panels