Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [ChIP-seq]

ABSTRACT: A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [ChIP-seq]

PROVIDER: PRJNA880538 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [SORT-seq]

Project description:Developmental signaling pathways associated with growth factors such as TGFb are commonly dysregulated in melanoma. Here we identified a human TGFb enhancer that was specifically activated in melanoma cells treated with TGFB1 ligand. We generated stable transgenic zebrafish with this enhancer driving green fluorescent protein (TIE:EGFP). EGFP was not expressed in normal melanocytes or early melanomas but was expressed in spatially distinct regions of mature melanomas. Single cell RNA-sequencing revealed that TIE:EGFP+ melanoma cells down-regulated interferon response, while up-regulating a novel set of chronic TGFb target genes. AP-1 factor binding is required for activation of this chronic TGFb reporter. Overexpression of the chromatin remodeler, SATB2, which is associated with tumor spreading shows activation of TGFb signaling in melanoma precursor zones and early melanomas. Confocal imaging and flow cytometric analysis showed that macrophages are recruited to EGFP positive regions and preferentially phagocytose TIE:EGFP+ cells. This work identifies a TGFb induced immune response and demonstrates the need for the development of chronic TGFb biomarkers to predict patient response to TGFb inhibitors.

2024-06-13 | GSE213359 | GEO

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [ChIP-seq]

2024-06-13 | GSE213326 | GEO

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, A375]

2024-06-13 | GSE213327 | GEO

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, ZMEL]

2024-06-13 | GSE213328 | GEO

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma

Project description:A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma

| PRJNA880593 | ENA

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [SORT-seq]

Project description:A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [SORT-seq]

| PRJNA880596 | ENA

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, ZMEL]

Project description:A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, ZMEL]

| PRJNA880540 | ENA

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, A375]

Project description:A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma [RNA-seq, A375]

| PRJNA880541 | ENA

A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-06-13 | GSE213360 | GEO

A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation [zebrafish ChIP-seq]

Project description:We report the distribution of H3K27Ac histone marks in a zebrafish melanoma cell line and a zebrafish primary melanoma tumor with the aim of identifying super-enhancer regions and regions of interest. We also report sox10 ChIP-Seq on the same zebrafish melanoma cell line.

2016-02-01 | GSE75353 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data