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Single cell analysis reveals the heterogeneity of fibroblasts in the diabetic heart

ABSTRACT: Single cell analysis reveals the heterogeneity of fibroblasts in the diabetic heart

PROVIDER: PRJNA880548 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21566208_1.fastq.gz	Fastqsanger.gz
	SRR21566208_2.fastq.gz	Fastqsanger.gz
	SRR21566209_1.fastq.gz	Fastqsanger.gz
	SRR21566209_2.fastq.gz	Fastqsanger.gz
	SRR21566210_1.fastq.gz	Fastqsanger.gz

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Bulk RNA sequecing reveals the heterogeneity of fibroblasts in the diabetic heart

Project description:Bulk RNA sequecing reveals the heterogeneity of fibroblasts in the diabetic heart

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Single cell analysis reveals the heterogeneity of fibroblasts in the diabetic heart

Project description:Diabetes mellitus is one of the most chronic diseases, of which diabetic cardiomyopathy is the major cause of morbidity and involves in multiple processes such as inflammation, oxidative stress, fibrosis, extracellular collagen deposition, apoptosis, mitochondria dysfunction. However, the exact mechanisms of fibroblasts concerning type Ⅰ diabetes remain unclear. To further understand the functional roles of fibroblasts of STZ-induced diabetic mice, we lead the single cell RNA sequence. Cells were comprised of endothelial, fibroblast, cardiomyocyte, smooth muscle cells, macrophage and other type cells. Single cell sequence illustrates novel fibroblast sub-clusters and highlight the role of Lox. Real-time quantitative PCR, western blotting, immunofluorescence was used to verify the sequence data. We validate the dysfunctions of diabetic cardiomyopathy by echocardiogram. Our study supports novel insights into the pathogenesis of diabetic cardiomyopathy.

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Bulk RNA sequecing reveals the heterogeneity of fibroblasts in the diabetic heart

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