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Sus scrofa

ABSTRACT: Sus scrofa neutrophils infected by Salmonella Typhimurium

PROVIDER: PRJNA881236 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21620802.fastq.gz	Fastqsanger.gz
	SRR21620803.fastq.gz	Fastqsanger.gz
	SRR21620804.fastq.gz	Fastqsanger.gz
	SRR21620805.fastq.gz	Fastqsanger.gz
	SRR21620806.fastq.gz	Fastqsanger.gz

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Total RNA sequencing in multiple Sus Scrofa tissues reveals novel long non-coding RNAs functioning in skeletal muscle development

Project description:Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes. However, the landscape of lncRNAs is largely unclear in Sus scrofa. Here we performed stranded RNA-seq on total RNA libraries from over 100 samples of Sus scrofa tissues. We identified 10,813 lncRNAs in Sus scrofa, of which 9,075 are novel. 57% of these lncRNAs were conserved in both human and mouse. These conserved lncRNAs tend to be more tissue-specific than pig-specific lncRNAs, and enriched in reproducible organs (i.e. testis and ovary). We characterized a group of lncRNAs potentially involved in the skeletal muscle development. One such lncRNA, a homolog of maternally expressed gene 3 (MEG3), was specifically expressed in the skeletal muscle at early developmental stage. And its expression pattern is conserved in pig and mouse. By over-expressing and knocking down MEG3 in mouse myoblast cell lines, we demonstrated its novel function as a myoblast proliferation suppressor.

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Project description:It has been proposed that chronic exposure to deoxynivalenol modulates hepatic gene expression and that sensivity to xenobiotics like deoxynivalenol is modulated by inflammation. We used microarrays to evaluate whether chronic deoxynivalenol exposure influences the hepatic gene expression of Sus scrofa in vivo and whether interaction between chronic deoxynivalenol exposure and systemic inflammation occur.

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