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Small RNA profiling of aster yellows infected Catharanthus roseus plants

ABSTRACT: Small RNA profiling of aster yellows infected Catharanthus roseus plants

PROVIDER: PRJNA882404 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21639529.fastq.gz	Fastqsanger.gz
	SRR21639530.fastq.gz	Fastqsanger.gz
	SRR21639531.fastq.gz	Fastqsanger.gz
	SRR21639532.fastq.gz	Fastqsanger.gz
	SRR21639533.fastq.gz	Fastqsanger.gz

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Small RNA profiling of aster yellows infected Catharanthus roseus plants

Project description:Aster yellows phytoplasma strain Hyd35 (16SrI-B) in micropropagated periwinkle shoots in collection was used to produce infected plants in pots that were separated according to the diverse symptomatology i.e. phyllody and witches’ broom. Small RNA high-throughput sequencing (HTS) was then used to determine the small RNA pattern of these plants. Bioinformatics analysis revealed the presence of expression changes of different miRNA classes and the presence of phytoplasma derived small RNAs. These results could complement previous studies and serve as a starting point for small RNA omics in phytoplasma research

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Project description:This dataset belongs to a set of three RNA-Seq experiments that were carried out to study the regulation of monoterpenoid indole alkaloid production in the medicinal plant Catharanthus roseus. For this dataset, C. roseus hairy roots overexpressing the well-known MIA biosynthesis regulator ORCA3 were analyzed by RNA-Seq. As control, C. roseus hairy roots expressing GUS were used. Each analyzed sample consisted of an independent hairy root line; three hairy root lines per construct were analyzed.

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