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ABSTRACT: Genome Assembly of Drosophila arizonae Chiapas Population

PROVIDER: PRJNA882912 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21665172_1.fastq.gz	Fastqsanger.gz
	SRR21665172_2.fastq.gz	Fastqsanger.gz
	SRR21665173_1.fastq.gz	Fastqsanger.gz
	SRR21665173_2.fastq.gz	Fastqsanger.gz
	SRR21665174_subreads.fastq.gz	Fastqsanger.gz

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Project description:We utilized three ecologically diverse Drosophila species to explore the influence of ecological adaptation on transcriptomic responses to isocaloric diets differing in their relative proportions of protein to sugar. Drosophila melanogaster, a cosmopolitan species that breeds in decaying fruit, exemplifies individuals long exposed to a Western diet higher in sugar, while the natural diet of the cactophilic D. mojavensis, is much lower in carbohydrates. Drosophila arizonae, the sister species of D. mojavensis, is largely cactophilic, but also utilizes rotting fruits that are higher in sugars than cacti. We exposed third instar larvae for 24 hours to diets either (1) high in protein relative to sugar, (2) diets with equal amounts of protein and sugar, and (3) diets low in protein but high in sugar. As we predicted, based upon earlier interspecific studies of development and metabolism, the most extreme differences in gene expression under different dietary conditions were found in D. mojavensis followed by D. arizonae. No differential expression among diets was observed for D. melanogaster, a species that survives well under all three conditions, with little impact on its metabolism. We suggest that these three species together provide a model to examine individual and population differences in vulnerability to lifestyle-associated health problems such as metabolic syndrome and diabetes.

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Project description:Whole genome sequencing of Drosophila arizonae and Drosophila mojavensis

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Project description:Ovaries transcriptome sequencing of Drosophila mojavensis, Drosophila arizonae and their hybrids

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Project description:Mycobacterium tuberculosis isolates from an amerindian population from Chiapas, Mexico

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Project description:In order to polish a long-read genome assembly, short-read illumina data was obtained from Heterodera schachtii cysts (Woensdrecht population from IRS, the Netherlands). Cysts where obtained from infected plant material. Nematodes were cleaned using a sucrose gradient centrifugation step. Thereafter DNA was extracted and used for library preparation and sequencing by Illumina NextSeq500.

2023-11-03 | E-MTAB-13472 | biostudies-arrayexpress

Project description:Ovaries and testes transcriptomes of Drosophila arizonae, Drosophila mojavensis mojavensis, Drosophila mojavensis wrigleyi and reciprocal hybrids

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2022-01-27 | PXD031165 | Pride

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