Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Key Genetic Determinants Driving Esophageal Squamous Cell Carcinoma Initiation and Immune Evasion [scRNAseq]

ABSTRACT: Key Genetic Determinants Driving Esophageal Squamous Cell Carcinoma Initiation and Immune Evasion [scRNAseq]

PROVIDER: PRJNA883203 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Key Genetic Determinants Driving Esophageal Squamous Cell Carcinoma Initiation and Immune Evasion [scRNAseq]

Project description:Accumulated genetic mutations or copy number alterations are frequently observed in esophageal squamous cell carcinoma (ESCC) patients. However, it is still elusive which gene is the driver to initiate ESCC. We identified key genetic determinants for ESCC development using CRISPR/Cas9-based multiple genes KO mouse esophageal organoid model. Trp53, Cdkn2a, and Notch1 triple KO (PCN) organoid showed the phenotypes of ESCC. These triple genes KO served for cell proliferation through building multiple root cell clusters and remodeling the immune landscape beneficial for tumorigenesis by Ccl2-Ccr2 mediated intercellular interactions. Ccl2-releasing PCN tumors were surrounded by exhausted T cells and M2 macrophages leading to immune evasion. The PCN-type tumor was observed in more than 30% of ESCC patients who express high levels of B2M, CCL2, and NF-kB. Our study unveiled genetic determinants for ESCC development crucial for cell-autonomous growth as well as non-cell autonomous interactions with immune cells.

2023-06-07 | GSE213927 | GEO

Key Genetic Determinants Driving Esophageal Squamous Cell Carcinoma Initiation and Immune Evasion

Project description:Key Genetic Determinants Driving Esophageal Squamous Cell Carcinoma Initiation and Immune Evasion

| PRJNA883199 | ENA

Key Genetic Determinants Driving Esophageal Squamous Cell Carcinoma Initiation and Immune Evasion [bulkRNAseq]

Project description:Key Genetic Determinants Driving Esophageal Squamous Cell Carcinoma Initiation and Immune Evasion [bulkRNAseq]

| PRJNA882909 | ENA

Key Genetic Determinants Driving Esophageal Squamous Cell Carcinoma Initiation and Immune Evasion [bulkRNAseq]

2023-06-07 | GSE213904 | GEO

Key Genetic Determinants Driving Esophageal Squamous Cell Carcinoma Initiation and Immune Evasion

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-06-07 | GSE213929 | GEO

Single cell RNA sequencing of thymic dendritic cells reveals molecular determinants of antigen transfer in the thymus

Project description:The presentation of self-antigens in the thymus by medullary thymic epithelial cells (mTECs) and dendritic cells (DCs) is crucial for the establishment of central tolerance. While mTECs produce and present self-antigens in an autonomous manner, DCs acquire them from mTECs by Cooperative Antigen Transfer (CAT). Although CAT represents a key process of central tolerance, molecular determinants which drive CAT are largely unknown. To reveal these determinants, we compared the transcriptomes of CAT-experienced and CAT-inexperienced DCs via scRNAseq. Specifically, we sequenced TdTOMATO+ and TdTOMATO– CD11c+ and CD11b+ cells from the thymus of Foxn1CreR26TdTOMATO mice. In this model, fluorescent TdTOMATO protein is exclusively produced by thymic epithelium, thus TdTOMATO expression in DCs (CD11c+ and CD11b+ cells) mark their previous experience of CAT. Using this approach we unraveled several candidate determinants of CAT. We focused on the gene determinant, Cldn1, encoding the tight junction protein Claudin 1, that was found to be highly expressed among the CAT-experienced type 1 lineage DCs. We performed further experiments that confirmed its role in driving CAT. In addition, this scRNAseq approach allowed us to redefine the heterogeneity and maturation states of thymic DCs.

2024-07-31 | E-MTAB-14319 | biostudies-arrayexpress

Hepatocytes coordinate immune evasion in cancer via release of serum amyloid A proteins [scRNAseq]

Project description:Hepatocytes coordinate immune evasion in cancer via release of serum amyloid A proteins [scRNAseq]

| PRJNA973601 | ENA

Identifying non-genetic determinants of malignant clonal fitness at single cell resolution [retransplant scRNAseq]

Project description:Identifying non-genetic determinants of malignant clonal fitness at single cell resolution [retransplant scRNAseq]

| PRJNA773263 | ENA

Identifying non-genetic determinants of malignant clonal fitness at single cell resolution [chemo scRNAseq]

Project description:Identifying non-genetic determinants of malignant clonal fitness at single cell resolution [chemo scRNAseq]

| PRJNA773261 | ENA

Molecular Determinants of Esophageal Cancer in Tanzania

Project description:Molecular Determinants of Esophageal Cancer in Tanzania