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Sewage Sludge

ABSTRACT: Microbial Communities and Sulfate-Reducing Microorganisms Abundance, Diversity and functional activity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plants

PROVIDER: PRJNA883522 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22212585_1.fastq.gz	Fastqsanger.gz
	SRR22212585_2.fastq.gz	Fastqsanger.gz
	SRR22212586_1.fastq.gz	Fastqsanger.gz
	SRR22212586_2.fastq.gz	Fastqsanger.gz
	SRR22212587_1.fastq.gz	Fastqsanger.gz

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Sewage Sludge

Project description:Microbial Communities and Sulfate-Reducing Microorganisms Abundance, Diversity and functional activity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plants

| PRJNA899302 | ENA

Global hepatic gene expression in rainbow trout exposed to sewage effluents

Project description:Effluents from sewage treatment plants contain a mixture of micropollutants with the potential of harming aquatic organisms. Thus, addition of advanced treatment techniques to complement existing conventional methods has been proposed. Some of the advanced techniques could, however, potentially produce additional compounds affecting exposed organisms by unknown modes of action. In the present study the aim was to improve our understanding of how exposure to different sewage effluents affects fish. This was achieved by explorative microarray and quantitative PCR analyses of hepatic gene expression, as well as relative organ sizes of rainbow trout exposed to different sewage effluents (conventionally treated, granular activated carbon, ozonation (5 or 15 mg/L), 5 mg/L ozone plus a moving bed biofilm reactor, or UV-light treatment in combination with hydrogen peroxide). Exposure to the conventionally treated effluent caused a significant increase in liver and heart somatic indexes, an effect removed by all other treatments. Genes connected to xenobiotic metabolism, including cytochrome p450 1A, were differentially expressed in the fish exposed to the conventionally treated effluents, though only effluent treatment with granular activated carbon or ozone at 15 mg/L completely removed this response. The mRNA expression of heat shock protein 70 kDa was induced in all three groups exposed to ozone-treated effluents, suggesting some form of added stress in these fish. The induction of estrogen-responsive genes in the fish exposed to the conventionally treated effluent was effectively reduced by all investigated advanced treatment technologies, although the moving bed biofilm reactor was least efficient. Taken together, granular activated carbon showed the highest potential of reducing responses in fish induced by exposure to sewage effluents.

2012-05-10 | GSE37574 | GEO

sludge metagenome

Project description:Microbiome of anaerobic digesters in municipal wastewater treatment plants

| PRJDB5190 | ENA

Exposure of Feathead Minnows to Municipal Wastewater Effluent Inhibits the Canonical Wnt Signaling Pathway in the Liver

Project description:Municipal wastewater effluent can impact its receiving environment. In the St. Lawrence River, male fish living downstream from Montreal exhibit increased hepatic vitellogenin, intersex, delayed spermatogenesis and altered immune function. Few studies have examined genome-wide effects associated with municipal effluent exposure in fish to decipher the mechanisms of toxicity. The present objective was to identify hepatic cellular signaling pathways in fathead minnows following exposure to municipal wastewater effluent. Immature minnows were exposed for 21 days to either 0% (Control) or 20% municipal effluent, the highest concentration in the St. Lawrence River. Hepatic RNA was extracted and used to hybridize a fathead minnow oligonucleotide microarray containing approximately 15K gene sequences.

2014-08-31 | GSE52693 | GEO

wastewater metagenome

Project description:Pyrosequencing reads from four municipal sewage wastewater treatment plants Metagenome

| PRJNA233577 | ENA

Hepatic gene expression in chinook salmon (Oncorhynchus tshawytscha) exposed to sewage

Project description:Hepatic transcriptional profiling of fish exposed to sewage to evaluate temporal and concentration trends.

2012-12-01 | GSE38925 | GEO

Discovery of resistant microorganisms in effluent from sewage treatment plants

Project description:Discovery of resistant microorganisms in effluent from sewage treatment plants

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Exposure of Feathead Minnows to Municipal Wastewater Effluent Inhibits the Canonical Wnt Signaling Pathway in the Liver

2014-08-31 | E-GEOD-52693 | biostudies-arrayexpress

Effects of TiO2 and Ag nanoparticles on polyhydroxybutyrate biosynthesis by activated sludge bacteria

Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers.

2014-12-03 | GSE63781 | GEO

Paracoccus denitrificans proteomics LC-MSMS

Project description:In response to the issues of low denitrification efficiency and high N₂O emissions in the biological nitrogen removal process of low C/N municipal wastewater, studies typically address these challenges by adding carbon sources. In this study, the addition of microorganisms enhanced the carbon flow and electron transport for nitrate reduction, significantly improving the denitrification performance of low C/N wastewater and reducing N₂O production. Proteomic analysis was employed to explore the mechanisms underlying this effect. The results revealed that the metabolites produced by the added microorganisms, S. oneidensis MR-1 and B. subtilis, including biosurfactants, heme, and cytochromes, altered the intracellular carbon redistribution in P. denitrificans, leading to an increased carbon flow directed towards nitrate reduction, thus enhancing total nitrogen removal efficiency.

2024-10-15 | PXD056799 |

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