Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CEBPD suppresses motility-associated gene signatures and reduces pancreatic ductal adenocarcinoma cell migration

ABSTRACT: CEBPD suppresses motility-associated gene signatures and reduces pancreatic ductal adenocarcinoma cell migration

PROVIDER: PRJNA883658 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21683948_1.fastq.gz	Fastqsanger.gz
	SRR21683948_2.fastq.gz	Fastqsanger.gz
	SRR21683949_1.fastq.gz	Fastqsanger.gz
	SRR21683949_2.fastq.gz	Fastqsanger.gz
	SRR21683950_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

CEBPD suppresses motility-associated gene signatures and reduces pancreatic ductal adenocarcinoma cell migration

Project description:To investigate the effects of CEBPD on pancreatic ductal cell tumorigenicity, we established doxycycline-inducible MIA PaCa-2 cells for CEBPD over-expression, as well as non-control cells with an empty inducible construct. RNAseq was performed on these cells in the presence and absence of doxycycline and we showed that CEBPD activates gene signatures associated with cell motility.

2022-09-26 | GSE214028 | GEO

Macrophage CEBPD dependent gene expression

Project description:RNAseq was performed on wild type, CEBPD knock out and CEBPD transactivation domain deleted RAW264.7 macrophages.

2021-10-06 | GSE173552 | GEO

MicroRNA-31 reduces the motility of proinflammatory T helper 1 lymphocytes

Project description:MicroRNA-31 reduces the motility of proinflammatory T helper 1 lymphocytes

| PRJNA504008 | ENA

Macrophage CEBPD dependent gene expression

Project description:Macrophage CEBPD dependent gene expression

| PRJNA726152 | ENA

Role of Cebpd on transcriptional output in 4T1 mouse triple negative breast cancer cells

Project description:Cebpd is a transcription factor regulating cytokines. We determined the role of Cebpd in the regulation of transciptional output. We generated 4T1 breast cancer cells with CRISPR/Cas9 mediated Cebpd knockout. We treated control sgRosa26 and sgCebpd cells with vehicle or IFNgamma (20ng/ml) for 24 hours and isolated RNA for sequencing.

2021-04-27 | GSE173296 | GEO

ANKRD1 promotes cell migration by suppressing ELN production during early senescence

Project description:Cellular senescence is a complex biological process that contributes to wound healing, carcinogenesis, and age-related disease. Although the molecular mechanisms whereby senescence promotes wound repair are not well understood, the protein ANKRD1, which promoted wound healing in mice, was found to increase in senescent cells. We hypothesized that ANKRD1 may play a role in senescence-mediated wound healing. Conditioned medium (CM) from senescent WI-38 human diploid fibroblasts hastened cell migration of human HaCaT keratinocytes. Interestingly, silencing ANKRD1 in WI-38 cells reduced the effect of CM on cell migration, while overexpressing ANKRD1 accelerated it. Further proteomic analysis revealed that ANKRD1 associates with YBOX1, a multifunctional protein that modulates transcription of the ELN gene and reduces ELN mRNA production. The product of the ELN gene, the protein ELN or tropoelastin (a subunit of elastin), is a secreted factor that reduces motility. Thus, we propose that a rise in ANKRD1 during early senescence transiently limits the YBOX1-dependent transcriptional increase in ELN production, and thereby enables cell motility in early phases of senescence.

2023-06-01 | MSV000092079 | MassIVE

Identify key factors regulated by transcription factor Cebpd in the kidney during heart regeneration in zebrafish

Project description:In previous studies, we identified and demonstrated that a 1.2kb cebpd downstream element (CEN) is capable and neccessary for the induction of cebpd in the kidney after cardiac injury. The goal of this study is to unveal the Cebpd regulated responses of kidney to cardiac regeneration in zebrafish. we profiled responses by kidney in cebpd mutant and enhancer (CEN) mutant fish zebrafish to massive injuries of the heart.

2022-02-25 | GSE193630 | GEO

MYBPH, a novel transcriptional target of NKX2-1/TTF-1, inhibits ROCK1 and actomyosin assembly, and reduces cell motility and tumor metastasis

Project description:Cell migration driven by actomyosin filament assembly is a critical step in tumour invasion and metastasis. Herein, we report identification of myosin binding protein H (MYBPH) as a transcriptional target of NKX2-1 (also known as TTF-1 and TITF1), a lineage-survival oncogene in lung adenocarcinoma. MYBPH inhibits assembly competence-conferring phosphorylation of the myosin regulatory light chain (RLC) as well as activating phosphorylation of LIM domain kinase (LIMK). These are unexpectedly implemented through direct physical interaction of MYBPH with Rho kinase 1 (ROCK1) rather than with RLC. In addition, MYBPH is shown to directly bind with non-muscle myosin heavy chain IIA (NMHC IIA), resulting in inhibition of NMHC IIA assembly. Thus, MYBPH plays multi-facetted roles in negative regulation of actomyosin organization, which in turn reduces cell motility, invasion, and metastasis. Finally, we also show that MYBPH is epigenetically inactivated by promoter DNA methylation in a fraction of lung adenocarcinomas abundantly expressing NKX2-1, which appears to be in accordance with its deleterious function for lung adenocarcinoma invasion and metastasis, as well as with the paradoxical association of NKX2-1 expression with favourable prognosis in lung adenocarcinoma patients. Dye-swap experiment, vector control vs. transiently transfectanted with TTF-1 in HPL1D, immortalized human peripheral lung epithelial cell line.

2011-12-22 | E-GEOD-26721 | biostudies-arrayexpress

Targeting the Epigenome Reduces Keloid Fibroblast Cell Proliferation, Migration and Invasion

Project description:Targeting the Epigenome Reduces Keloid Fibroblast Cell Proliferation, Migration and Invasion

| PRJNA970758 | ENA

Effect of Hsp70 inhibitor JG-98 on gene expression in mouse macrophages

Project description:JG-98 reduces migration of macrophages. We assessed how this compound affects expression of genes associated with motility and migration. A number of motility/migration genes were significantly downregulated.

2016-12-19 | GSE82311 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data