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Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis [RNA-seq]

ABSTRACT: Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis [RNA-seq]

PROVIDER: PRJNA884277 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21708476_1.fastq.gz	Fastqsanger.gz
	SRR21708476_2.fastq.gz	Fastqsanger.gz
	SRR21708477_1.fastq.gz	Fastqsanger.gz
	SRR21708477_2.fastq.gz	Fastqsanger.gz
	SRR21708478_1.fastq.gz	Fastqsanger.gz

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Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis [RNA-seq]

Project description:ZMYM2 is a transcriptional repressor whose role in development is largely unexplored. We found that Zmym2-/- mice show embryonic lethality by E10.5. Molecular characterization of Zmym2-/- embryos revealed two distinct defects. First, they fail to undergo DNA methylation and silencing of germline gene promoters, resulting in widespread upregulation of germline genes. Second, they fail to methylate and silence the evolutionarily youngest and most active LINE element subclasses in mice. This results in ubiquitous overexpression of LINE1 protein as well as aberrant expression of transposon-gene fusion transcripts. Interaction and colocalization data indicate that ZMYM2 homes to germline genes via binding to the non-canonical polycomb complex PRC1.6 and to transposons via the TRIM28 complex. ZMYM2-/- human embryonic stem cells also show aberrant upregulation and demethylation of young LINE elements, indicating a conserved role in repression of active transposons. ZMYM2 is thus an important new factor in DNA methylation patterning in early embryogenesis.

2023-06-14 | GSE214161 | GEO

Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis [WGBS-seq]

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Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis [ChIP-seq]

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Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis

Project description:Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis

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Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis [ChIP-seq]

Project description:Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis [ChIP-seq]

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Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis [WGBS-seq]

Project description:Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis [WGBS-seq]

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Zmym2 is essential for methylation of germline genes and active transposons in embryogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

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Maternally inherited piRNAs silence transposons during Drosophila embryogenesis

Project description:The piRNA pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. piRNAs are maternally deposited and required for proper transposon silencing in adult offspring. However, a long-standing question in the field is the precise function of maternally deposited piRNAs and its associated factors during embryogenesis. Here, we probe the spatio-temporal expression patterns of several piRNA pathway components during early stages of development. Amongst those, factors required for transcriptional gene silencing (TGS) showed ubiquitous abundance in somatic and pole cells throughout the first half of embryogenesis. We further analysed the transcriptomes of various embryo stages and correlated these with the presence of selected chromatin marks. We found that a number of transposon families show bursts of transcription during early embryonic stages. Transposons heavily targeted by maternally deposited piRNAs accumulated repressive chromatin marks following their spike in expression. Furthermore, depletion of maternally deposited Piwi protein in early embryos resulted in increased expression of transposons targeted by inherited piRNAs and was accompanied by a strong loss of repressive chromatin marks at coding sequences. Overall, our data suggests a pivotal role for the piRNA pathway in transposon defence during Drosophila embryogenesis in somatic cells.

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Maternally inherited piRNAs direct transient heterochromatin formation at active transposons during early Drosophila embryogenesis

Project description:The piRNA pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. piRNAs are maternally deposited and required for proper transposon silencing in adult offspring. However, a long-standing question in the field is the precise function of maternally deposited piRNAs and its associated factors during embryogenesis. Here, we probe the spatio-temporal expression patterns of several piRNA pathway components during early stages of development. Amongst those, factors required for transcriptional gene silencing (TGS) showed ubiquitous abundance in somatic and pole cells throughout the first half of embryogenesis. We further analyzed the transcriptomes of various embryo stages and correlated these with the presence of selected chromatin marks. We found that a number of transposon families show bursts of transcription during early embryonic stages. Transposons heavily targeted by maternally deposited piRNAs accumulated repressive chromatin marks following their spike in expression. Furthermore, depletion of maternally deposited Piwi protein in early embryos resulted in increased expression of transposons targeted by inherited piRNAs and was accompanied by a strong loss of repressive chromatin marks at coding sequences. Overall, our data suggests a pivotal role for the piRNA pathway in transposon defence during Drosophila embryogenesis in somatic cells.

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