Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

YAP1 is essential for self-organized differentiation of pluripotent stem cells [RNAseq]

ABSTRACT: YAP1 is essential for self-organized differentiation of pluripotent stem cells [RNAseq]

PROVIDER: PRJNA885143 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21743269.fastq.gz	Fastqsanger.gz
	SRR21743270.fastq.gz	Fastqsanger.gz
	SRR21743271.fastq.gz	Fastqsanger.gz
	SRR21743272.fastq.gz	Fastqsanger.gz
	SRR21743273.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 13

Similar Datasets

YAP1 is essential for self-organized differentiation of pluripotent stem cells

Project description:YAP1 is essential for self-organized differentiation of pluripotent stem cells

| PRJNA885138 | ENA

YAP1 is essential for self-organized differentiation of pluripotent stem cells [DNAm]

Project description:YAP1 is essential for self-organized differentiation of pluripotent stem cells [DNAm]

| PRJNA885141 | ENA

YAP1 is essential for self-organized differentiation of pluripotent stem cells [RNAseq]

Project description:The Yes-associated protein 1 (YAP1) is a downstream effector of the Hippo pathway and essential mechanotransducer. It has been suggested to play a crucial role for early embryo development, but the relevance for early germ layer commitment of human induced pluripotent stem cells (iPSCs) remains largely unclear. To gain better insight into the functional relevance of YAP1 in these early cell-fate decisions, we generated iPSC lines with YAP1 knockout (YAP-/-) with CRISPR/Cas9 technology and analyzed transcriptomic and epigenetic modifications. In YAP-/- iPSCs the expression of several YAP1 targets changed and NODAL, which is an important regulator of cell differentiation, was upregulated. Furthermore, YAP1 deficiency evoked global DNA methylation changes. Directed differentiation of adherent iPSC colonies toward endoderm, mesoderm, and ectoderm could be induced, albeit endodermal and ectodermal differentiation showed transcriptomic and epigenetic changes in YAP-/- lines. Notably, in self-organized embryoid bodies (EBs) germ layer specification was clearly impaired. This phenotype was rescued via lentiviral overexpression of YAP1 and in tendency also by NODAL inhibitors. Our results demonstrate that YAP1 plays an important role during early germ layer specification of iPSCs, particularly for the non-directed self-organization of EBs, and this is at least partly attributed to activation of the NODAL pathway.

2023-02-17 | GSE214401 | GEO

YAP1 is essential for self-organized differentiation of pluripotent stem cells [DNAm]

2023-02-17 | GSE214400 | GEO

YAP1 is essential for self-organized differentiation of pluripotent stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-17 | GSE214403 | GEO

MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells

Project description:MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells

| PRJNA634099 | ENA

MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells

Project description:Deciphering the mechanisms that control the pluripotent ground state is key for understanding embryonic development. Nonetheless, the epigenetic regulation of ground-state mouse embryonic stem cells (mESCs) is not fully understood. Here, we identify the epigenetic protein MPP8 as being essential for ground-state pluripotency. Its depletion leads to cell cycle arrest and spontaneous differentiation. MPP8 has been suggested to repress LINE1 elements by recruiting the human silencing hub (HUSH) complex to H3K9me3-rich regions. Unexpectedly, we find that LINE1 elements are efficiently repressed by MPP8 lacking the chromodomain, while the unannotated C-terminus is essential for its function. Moreover, we show that SETDB1 recruits MPP8 to its genomic target loci, whereas transcriptional repression of LINE1 elements is maintained without retaining H3K9me3 levels. Taken together our findings demonstrate that MPP8 protects the DNA-hypomethylated pluripotent ground state through its association with the HUSH core complex, however, independently of detectable chromatin binding and maintenance of H3K9me3.

2021-03-21 | PXD019345 | Pride

Self-organized trunk-like-structures with somites and a neural tube

Project description:Self-organized trunk-like-structures with somites and a neural tube

| PRJNA592534 | ENA

Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids

Project description:Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids

| PRJNA762700 | ENA

Self-Organized Generation Of Hepatic Cord Like Organoids (HCLOs) With Zonal Diversity

Project description:Self-Organized Generation Of Hepatic Cord Like Organoids (HCLOs) With Zonal Diversity

| PRJNA922964 | ENA