Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Anti-MN1 sdAb screening and its function in vivo

ABSTRACT: Anti-MN1 sdAb screening and its function in vivo

PROVIDER: PRJNA885857 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Anti-MN1 sdAb screening and its function in vivo

Project description:To study the function of anti-MN1 sdAb in MN1-positive tumour cells, we treated U2OS cells with anti-MN1 sdAb and performed RNA-seq.

2022-10-30 | GSE214561 | GEO

RNA-seq analysis of Men1-/- versus Men1wt MN1-driven AML

Project description:Purpose: To characterize transcriptional changes associated with homozygous inactivation of Men1 in MN1 driven AML Methods: In vivo MN1 transformed cells were transduced with Cre-vector to inactivate Men1 and injected into sublethially irradiated syngeneic recipients. Men1-/- and Men1wt MN1-driven leukemic cells were isolated from moribund recipients and plated in methylcellulose for 7 days before RNA was extracted. Results: Men1-/- MN1-driven leukemic cells failed to propagate leukemia in most secondary recipients, in comparison with Men1wt MN1-driven. In vitro, cells had lost their colony forming activity. Gene expression analysis revealed a downregulation of the MN1 leukemic expression program. Conclusions: Men1 regulates long term maintenance of MN1-driven leukemia.

2020-05-09 | GSE150151 | GEO

Functional dissection of Bmi1 independence of MN1 leukemia

Project description:MN1 leukemia is a poor prognosis leukemia occuring as MN1 overexpression or fusion with TEL (MN1-TEL), MN1 and MN1-TEL show different biology in terms of dependence of known self-renewal associated genes in leukemia, c-kit positive murine primary bone marrow cells were retrovirally transduced with MN1, MN1-TEL or MN1-TEL mutant MN1-TELdelDBD showing biological similarity with MN1, after retroviral transformation of cells and 10 d culture RNA was extracted and gene expression profiling was assessed

2016-08-12 | GSE50877 | GEO

RNA-seq of murine HSPCs overexpressing MN1 or MN1-∆pQ

Project description:Transcriptional effects of MN1 polyQ-stretch deletion in murine HSPCs

2020-07-24 | GSE154997 | GEO

In vivo Genome-scale CRISPR-Cas9 knockout screening

Project description:To identify essential gene responding to anti-PD-1 immunotherapy, we performed in vivo Genome-scale CRISPR-Cas9 knockout screening. We found that cohesin complex invovled in regulation of anti-PD-1 immunotherapy.

2022-01-14 | GSE125201 | GEO

RNA-seq of murine HSPCs overexpressing MN1 or MN1-∆pQ

Project description:RNA-seq of murine HSPCs overexpressing MN1 or MN1-∆pQ

| PRJNA648119 | ENA

MN1 or MN1-TEL in the promonocytic cell line U937

Project description:Expression profiling of U937 derived cell lines with induced expression of MN1or MN1-TEL in combination with all-trans retinoic acid (ATRA) Keywords: expression profiling Two similar experiments (A and B, biological duplicates) were performed. Hybridization includes dye swaps. See experimental_design.jpg (below) for detailed setup of the study. In short, different time points after induction of MN1 or MN1-TEL were compared to uninduced samples. The effects of all-trans retinoic acid (ATRA) were also investigated

2008-09-09 | E-GEOD-11441 | biostudies-arrayexpress

Gene expression changes induced by overexpression of MN1 and MN1VP16 in murine bone marrow cells

Project description:Myeloid differentiation is blocked in acute myeloid leukemia (AML), but the molecular mechanisms are not well characterized. MN1 is overexpressed in some AML patients and confers resistance to all-trans retinoic acid (ATRA)-induced differentiation. To understand the role of MN1 as a transcriptional regulator in myeloid differentiation, we fused transcriptional activation (VP16) or repression (M33) domains with MN1 and characterized these cells in vivo. Transcriptional activation of MN1 target genes induced myeloproliferative disease with long latency and differentiation potential to mature neutrophils. A large proportion of differentially expressed genes between leukemic MN1 and differentiation-permissive MN1VP16 cells belonged to the immune response pathway like Irf8 and Ccl9. As MN1 is a co-factor of MEIS1 and RARA, we compared chromatin occupancy between MN1, MEIS1 and RARA. Immune response genes that were upregulated in MN1VP16 cells were co-targeted by MN1 and MEIS1, but not RARA, suggesting that myeloid differentiation is blocked through transcriptional repression of shared target genes of MN1 and MEIS1. Constitutive expression of Irf8 or its target gene Ccl9 identified these genes as potent inhibitors of MN1-induced leukemia. Our data show that MN1 prevents activation of the immune response pathway, and suggest that restoration of Irf8 signalling as a novel therapeutic target in AML.

2015-01-01 | GSE48819 | GEO

MN1 or MN1-TEL in the promonocytic cell line U937

Project description:Expression profiling of U937 derived cell lines with induced expression of MN1or MN1-TEL in combination with all-trans retinoic acid (ATRA) Keywords: expression profiling

2008-09-09 | GSE11441 | GEO

MN1 colocalizes with the BAF complex on enhancer chromatin

Project description:We show that the overexpression of MN1, but not MN1-∆pQ, stabilizes the porogenitor-specific enhancer landscape

2020-07-17 | GSE154602 | GEO