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Effect of ICSI on gene expression in germline stem cells

ABSTRACT: Effect of ICSI on gene expression in germline stem cells

PROVIDER: PRJNA886622 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21784964.fastq.gz	Fastqsanger.gz
	SRR21784965.fastq.gz	Fastqsanger.gz
	SRR21784966.fastq.gz	Fastqsanger.gz
	SRR21784967.fastq.gz	Fastqsanger.gz
	SRR25918343.fastq.gz	Fastqsanger.gz

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Effect of ICSI on gene expression in germline stem cells

Project description:To investigate the effect of ICSI in germ cells, we performed RNA-seq of germline stem cells established from ICSI-derived mice.

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Project description:To investigate the effect of ICSI in germ cells, we performed RRBS of germline stem cells established from ICSI-derived mice.

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Regulation of germline gene expression in C. elegans germline stem cells.

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Effect of intra-cytoplasmic sperm injection (ICSI) on gene expression and development of mouse preimplantation embryos

Project description:Background: In vitro culture of preimplantation mouse embryos is associated with changes in gene expression. It is however not known if the method of fertilization affects the global pattern of gene expression. Method: We compared gene expression and development of mouse blastocysts produced by intra-cytoplasmic sperm injection and cultured in Whitten’s medium (ICSIWM) or KSOM medium with amino acids (ICSIKSOMaa) with control blastocysts flushed out of the uterus on post coital day 3.5 (In vivo). Global pattern of gene expression was assessed using the Affymetrix 430 2.0 chip. In addition we compared gene expression in embryos generated in IVF or ICSI using WM. Results: Blastocysts resulting from ICSI fertilization have a reduction in the number of trophoblastic and inner cell mass cells compared to in vivo generated embryos. Approximately 1000 genes are different between ICSI blastocyst and in vivo blastocysts; proliferation, apoptosis and morphogenetic pathways are the most common pathways altered after in vitro culture. Unexpectedly, only 41 genes were statistically different between embryo cultured in suboptimal conditions (WM) or optimal conditions (KSOMaa). Conclusion: The method of fertilization plays a more important role in shaping the transcriptome of the developing mouse embryo than the culture media used.

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Differential Gene Expression of Human Cumulus Granulosa and Mural Granulosa Cells in ICSI Patients

Project description:Differential Gene Expression of Human Cumulus Granulosa and Mural Granulosa Cells in ICSI Patients

2021-02-10 | GSE110924 | GEO

mRNA expression data from monkey PG-haESCs, ICSI-derived ESCs and somatic fibroblasts

Project description:Haploid cells are amenable for genetic analysis because they contain only one set of chromosomes. Here,we report the derivation of haESCs from monkey parthenogenic blastocysts. These cells, which we designated PG-haESCs (parthenogenic haploid embryonic stem cells), express classical ESC markers, are pluripotent, and can differentiate to different cell lines from all three embryonic germ layers in vivo and in vitro. We used microarrays to compare the gene expression levels among PG-haESC, ICSI-derived ESCs and female monkey somatic fibroblasts. We used ICSI-derived ESCs and somatic fibroblasts isloated from female individuals as control. Gene expression profiles of all the cell lines were analysed on an Affymetrix Rhesus Macaque array.

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Sequencing of proteomic pan-β-hydroxybutyrylation in female germline stem cells

Project description:Metformin increase the number of FGSCs in mouse ovary, and the increased pan-β-hydroxylbutylated protein modification level in vitro FGSCs was detected by WB. There are the Mass spectrum original data of proteomic examination about pan-β-hydroxybutyrylation sites in female germline stem cells after merformin treatment

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Chromatin and gene expression changes during female Drosophila germline stem cell development.

Project description:The maintenance and differentiation of highly potent animal stem cells generates an epigenetic cycle that underlies development. Drosophila female germline stem cells (GSC) produce cystoblast daughters that differentiate into nurse cells and oocytes. Developmental chromatin analysis profiling the differentiation of GSCs into cystoblasts and NCs of increasing ploidy shows that cystoblasts start developing by forming heterochromatin while in a transient syncytial state, the germline cyst, reminiscent of early embryonic cells. The open GSC chromatin state is further restricted by Polycomb repression of targets that include testis expressed genes briefly active in early female germ cells. Like other highly potent stem cells, GSC metabolism is reprogrammed and Myc-dependent growth is upregulated by altering mitochondrial membrane transport, gluconeogenesis and other processes. Thus, the animal generational cycle comprises similar but distinct maternal and zygotic stem cell epigenetic cycles. We propose that the pluripotent stem cell state and daughter cell differentiation were shaped by the pressure to resist transposon activity over evolutionary time scales. In this GEO submission, we present data and analyses pertaining to H3K27ac, H3K27me3, and H3K9me3 ChIPseq, ATACseq, and RNAseq of Germline Stem Cells (GSCs) and Nurse Cells (NCs) from Drosophila melanogaster ovaries.

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