Caenorhabditis briggsae SDC-2 and DPY-27 ChIP-seq
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ABSTRACT:
PROVIDER: PRJNA886730 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:ChIP-seq for the SDC-2 and DPY-27 subunits of the dosage compensation complex in C. briggsae
2023-02-15 | GSE214714 | GEO
Project description:Caenorhabditis briggsae Raw sequence reads
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Project description:DCC binding and function (ChIP-chip: SDC-3, DPY-27, Mock)
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Project description:This SuperSeries is composed of the following subset Series: GSE14649: DCC binding and function (Expression Analysis) GSE14650: DCC binding and function (ChIP-chip: SDC-3, MIX-1, DPY-27, Mock) GSE14651: DCC binding and function (ChIP-chip: DPY-27) GSE14652: DCC binding and function (ChIP-chip: SDC-2) GSE14653: DCC binding and function (ChIP-chip: SDC-3, DPY-27, Mock) Refer to individual Series
2010-05-22 | E-GEOD-14640 | biostudies-arrayexpress
Project description:DCC binding and function (ChIP-chip: SDC-3, MIX-1, DPY-27, Mock)
| PRJNA114439 | ENA
Project description:Examination of DPY-30, DPY-27, SDC-3, DPY-26, DPY-28, SDC-2, and SUMOylated DPY-27 binding in wild type embryos and smo-1 RNAi treated embryos
| PRJNA209991 | ENA
Project description:The essential process of dosage compensation equalizes X-chromosome gene expression between C. elegans XO males and XX hermaphrodites through a dosage compensation complex (DCC) that resembles condensin. The DCC binds to both X chromosomes of hermaphrodites to repress transcription by half. Here we show that post-translational modification by the SUMO conjugation pathway is essential for sex-specific assembly of the DCC onto X. Depletion of the SUMO peptide in vivo severely disrupts binding of particular DCC subunits and causes changes in X-linked gene expression similar to those caused by disrupting genes encoding DCC subunits. Three DCC subunits are themselves SUMOylated, and depletion of SUMO preferentially reduces their binding to X, suggesting that SUMOylation of DCC subunits is essential for robust association with X. DCC SUMOylation is triggered by the signal that initiates DCC assembly onto X. The initial step of assembly--binding of X-targeting factors to recruitment sites on X (rex sites)--is independent of SUMOylation, but robust binding of the complete complex requires SUMOylation. SUMOylated DCC subunits are enriched at rex sites, and SUMOylation enhances interactions between X-targeting factors and condensin subunits that facilitate DCC binding beyond the low level achieved without SUMOylation. DCC subunits also participate in condensin complexes essential for chromosome segregation, but their SUMOylation occurs only in the context of the DCC. Our results reinforce a newly emerging theme in which multiple proteins of a complex are SUMOylated in response to a specific stimulus, leading to accelerated complex formation and enhanced function. ChIP-chip experiments using antibodies against DPY-27, SDC-3, DPY-30, DPY-26, DPY-28 and FLAG-tagged SDC-2 in wild-type and smo-1 RNAi treated mixed embryos, with IGG controls. Also, sequential ChIP-chip experiments: (1) ChIP using FLAG antibodies to determine the genome-wide binding sites for SUMOylated proteins, (2) ChIP using FLAG antibodies followed by re-ChIP of eluted protein-chromatin complexes with DPY-27 antibodies to determine genome-wide binding sites for SUMOylated DPY-27 (referred to as DPY-27 re-ChIP experiments), (3) ChIP using FLAG antibodies followed by re-ChIP of eluted protein-chromatin with IGG antibodies to determine background binding (referred to as IGG re-ChIP experiments, and (4) ChIP using DPY-27 antibodies as a control to assess the efficiency of DPY-27 binding and detection in control vs. FLAG-tagged strains.
2013-08-31 | E-GEOD-48413 | biostudies-arrayexpress