Project description:We sequenced and analyzed the genome of a highly inbred miniature Chinese pig strain, the Banna Minipig Inbred Line (BMI). we conducted whole genome screening using next generation sequencing (NGS) technology and performed SNP calling using Sus Scrofa genome assembly Sscrofa11.1.
Project description:The severe inflammatory response of pulmonary epithelial cells is the main pathological feature of pneumonia caused by methicillin-resistant Staphylococcus aureus (MRSA). Zhenqi granules (ZQ), a compound herbal formula composed of Astragalus membranaceus and Ligustrum lucidum, has potential therapeutic effects on this inflammatory response, but its mechanism of action is still unclear. The study aims to explore the effect and mechanism of ZQ on the inflammatory response induced by establishing an in vitro model of MRSA-infected human pulmonary bronchial epithelial cells (MRSA-BEAS-2B). ZQ (10 mg/mL) had no inhibitory effect on MRSA or its biofilm, but it reduced the levels of LDH and c-di-AMP in MRSA-BEAS-2B cells. The mechanism is attributed to the downregulation of endoplasmic reticulum-related protein pathways, specifically involving a decrease in endoplasmic reticulum stress-related proteins such as STING and ERAdP; a decrease in inflammation pathway-related factors such as NF-ĸB, IFR3, NLRP3, and TLR4; and an increase in the STING antagonist RECON. These effects resulted in a reduction in the proinflammatory cytokines IFN-β, IL-1β, IL-6, and IL-18, thereby inhibiting pulmonary inflammation. ZQ attenuates the inflammatory response of pulmonary epithelial cells by inhibiting c-di-AMP-related endoplasmic reticulum stress during MRSA infection. This study provides a basis for the clinical application of ZQ and improves the development of treatment strategies for pulmonary MRSA infection.
Project description:Leishmania donovani WHO reference strain MHOM/IN/80/DD8 and Leptomonas seymouri isolates Ld 2001 and Ld39 were used for proteome analysis which were originally isolated from clinical cases of kala azar patients with different inherent antimonial sensitivities. Ld 2001 was Sb-S and Ld 39 was Sb-R. The genome sequencing of these isolates had confirmed co-infection with Leptomonas.
Project description:Candida lusitaniae is an emerging human opportunistic yeast, which can switch from yeast to pseudohyphae, and one of the rare Candida species capable of sexual reproduction. Its haploid genome and the genetic tools available make it a model of interest to study gene function. This study describes the consequences of DPP3 inactivation on cell morphology and mating, both altered in the dpp3Δ knock-out. Interestingly, reintroducing a wild-type copy of the DPP3 gene in the dpp3Δ mutant failed to restore the wild-type phenotypes. Proteomic analyses showed that about 150 proteins were statistically deregulated in the dpp3Δ mutant, and that most of them did not return to their wild-type level in the reconstituted DPP3 strain. The analysis of the segregation of the dpp3Δ mutation and the phenotypes in the progeny of a cross (between the dpp3Δ knock-out and a wild-type strain) showed that the phenotypes are not linked to dpp3Δ, but to a secondary mutation. Genome sequencing of the dpp3Δ mutant allowed us to identify this secondary mutation.
