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Self-organizing in vitro mouse neural tube organoids mimic embryonic development

ABSTRACT: Self-organizing in vitro mouse neural tube organoids mimic embryonic development

PROVIDER: PRJNA888056 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21837684_1.fastq.gz	Fastqsanger.gz
	SRR21837684_2.fastq.gz	Fastqsanger.gz
	SRR21837685_1.fastq.gz	Fastqsanger.gz
	SRR21837685_2.fastq.gz	Fastqsanger.gz
	SRR21837686.fastq.gz	Fastqsanger.gz

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Self-organizing in vitro mouse neural tube organoids mimic embryonic development

Project description:Self-elongating neural tube organoids recapitulate key aspects of the morphology, anterior-posterior patterning, neural crest emergence and neural differentiation of mouse embryo in vivo by self-organization. We used single-cell RNA sequencing (scRNA-seq) to analyse the cell types and to reveal the sequence of transcriptional events in the emergence of neural crest cells and neural differentiation.

2022-10-12 | GSE214996 | GEO

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Project description:Self-organizing in vitro neural tube organoids mimic embryonic development

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Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition

Project description:Organoids can undergo pattern formation by spontaneously forming spatially-localised signaling centers called organisers. Understanding how molecular pathways participate in this regulative process is a central challenge in biology. Here, we investigated self-organisation of a SHH-expressing floorplate in clonal neural tube organoids (NTOs). In NTOs, a pulse of retinoic acid (RA) applied at Day 2 triggers self-organisation of a FOXA2+ floorplate by Day 6. FOXA2 expression was initially spatially scattered before resolving into multiple clusters. These FOXA2+ clusters underwent competition and physical sorting, resulting in a stable “winning” floorplate. We identified BMP signalling as a key governor of long-range cluster competition.

2024-04-23 | GSE236543 | GEO

Self-organizing Single-Rosette Brain Organoids (SOSRS) from Human Pluripotent Stem Cells

Project description:Self-organizing Single-Rosette Brain Organoids (SOSRS) from Human Pluripotent Stem Cells

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Self-organizing human trunk 3D neuromuscular organoids

Project description:Neuromuscular networks assemble during early human embryonic development and are essential for the control of body movement. Previous neuromuscular junction modeling efforts using human pluripotent stem cells (hPSCs) generated either spinal cord neurons or skeletal muscles in monolayer culture. Here, we use hPSC-derived axial stem cells, the building blocks of the posterior body, to simultaneously generate spinal cord neurons and skeletal muscle cells that self-organize to generate human neuromuscular organoids (NMOs) that can be maintained in 3D for several months. Single-cell RNA-sequencing of individual organoids revealed reproducibility across experiments and enabled the tracking of the neural and mesodermal differentiation trajectories as organoids developed and matured. NMOs contain functional neuromuscular junctions supported by terminal Schwann cells. They contract and develop central pattern generator-like neuronal circuits. Finally, we successfully use NMOs to recapitulate key aspects of myasthenia gravis pathology, thus highlighting the significant potential of NMOs for modeling neuromuscular diseases in the future.

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Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition

Project description:Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition

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Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition

Project description:Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition

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Effect of Trp53 knockout on fallopian tube epithelial organoids

Project description:We established murine fallopian tube epithelial organoids from a B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J mouse. Subsequently, we knocked out Trp53 by introducing sgRNA into the organoids, nutlin-3 selection, and single-organoid cloning. The Trp53-knocked organoids grew faster than the normal organoids. We also analyzed the transcriptomic differences caused by Trp53-knockout by RNA-sequencing and gene set enrichment analysis (GSEA), which indicated that Trp53-knockout reduced cilium-related gene expression. In human HGSC precancerous lesions, such as serous tubal intraepithelial carcinoma (STIC), differentiation to ciliated cells has been reported to be down-regulated; therefore, the genetic manipulation was supposed to mimic the process of carcinogenesis of HGSC.

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