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Expression data from human lung epithelial A549 cells treated with DMEM containing 5.5 or 25 mM

ABSTRACT: Expression data from human lung epithelial A549 cells treated with DMEM containing 5.5 or 25 mM

PROVIDER: PRJNA889325 | ENA |

REPOSITORIES: ENA

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Expression data from human lung epithelial A549 cells treated with DMEM containing 5.5 or 25 mM

Project description:Cells treated with DMEM containing 25 mM had higher cellular translation levels than that of cells treated with DMEM containing 5.5 mM . We used microarrays to detail the global gene expression underlying hyperglycemic stress.

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Transcriptomic data of MCF7 cells adapted to culture in media containing different sugars (glucose or fructose) and cultured in 2D on plastic

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Transcriptomic data of MDA-MB-231 cells adapted to culture in media containing 0 mM or 2 mM metformin and cultured as mammospheres

Project description:We report the single-cell RNA sequencing data obtained from MDA-MB-231 breast cancer cells cultured in standard DMEM (high glucose) media, or adapted to culture in standard DMEM (high glucose) media containing 2 mM metformin, and then cultured as mammospheres

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DMEM treated WT and PKBa -/- MEFs

Project description:Mouse embryonic fibroblasts (MEFs) were generated from 13.5-day-old embryos obtained from heterozygous PKBa mice intercrosses (Yang et al., 2003). Briefly, after dissection of head and visceral organs for genotyping, embryos were minced and trypsinized for 30 min at 37°C. Embryonic fibroblasts were then plated and maintained in Dulbecco’s Modified Eagle Medium (DMEM) with 10% foetal calf serum (FCS) (Life Technologies), 100 units/ml of penicillin and 100 mg/ml of streptomycin at 37°C in an atmosphere of 5% CO2. All experiments were performed with wild-type and PKBa-/- MEFs between 15-20 passages. To induce adipocyte differentiation, 2-day-postconfluent cells (day 0) were treated with DMEM supplemented with 10% FCS, 8 mg/ml biotin, 4 mg/ml pantothenate, 0.5 mM 3-isobutyl-1-methylxanthine, 1 mM dexamethasone and 10 mg/ml insulin (all from Sigma). Total RNA was extracted from cells using TRIzol (Invitrogen) according to the manufacturer’s instructions. Keywords: repeat

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Thrombospondin-1: A Pro-Atherosclerotic Protein Augmented by Hyperglycemia

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Effect of restricted glycolysis on gene expression in MDA-MB-231 cells

Project description:Cultured breast cancer cells in standard media are highly glycolytic To determine the effect of glycolysis on gene expression in MDA-MB-231 we adapted cells to cuture in mediae containing 10 mM fructose, and compared the gene expression to cells cutured in standard high glucse (25 mM) DMEM

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Effect of restricted glycolysis on gene expression in MCF-7 cells

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