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Investigation of the direct activity of type I interferon on Ifnar1 KO ID8 ovarian cancer cells in vitro.

ABSTRACT: Investigation of the direct activity of type I interferon on Ifnar1 KO ID8 ovarian cancer cells in vitro.

PROVIDER: PRJNA889445 | ENA |

REPOSITORIES: ENA

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Investigation of the direct activity of type I interferon on Ifnar1 KO ID8 ovarian cancer cells in vitro.

Project description:This experiment is part of a larger study examining the anti-tumour properties of interferon epsilon in ovarian cancer. The goal of this experiment was to assess whether interferon signalling had been ablated in Ifnar1 CRISPR knockout (KO) ID8 cell lines, so that they could be used for in vivo models of ovarian cancer.

2023-03-28 | GSE215261 | GEO

Investigation of the direct activity of interferon epsilon on ID8 ovarian cancer cells in vitro.

Project description:This experiment is part of a larger study examining the anti-tumour properties of interferon epsilon in ovarian cancer. The objective of this experiment was to examine the direct activity of interferon epsilon on the mouse ovarian cancer cell line, ID8, and compare it to equivalent unit concentrations of interferon beta. The goal was to determine whether interferon epsilon and interferon beta induce different patterns of gene expression in ID8 cells.

2023-03-28 | GSE201345 | GEO

Investigation of the direct activity of interferon epsilon on ID8 ovarian cancer cells in vitro.

Project description:Investigation of the direct activity of interferon epsilon on ID8 ovarian cancer cells in vitro.

| PRJNA830905 | ENA

Structural basis of a novel Interferon b signaling axis mediated via the ifnar1 receptor

Project description:Type I interferons (IFNs) are a family of cytokines that play an important role in regulating immune responses to pathogens and tumors and are used therapeutically. All IFNs are considered to signal via the heterodimeric IFNAR1-IFNAR2 complex, yet some subtypes such as IFN? can exhibit distinct functional properties, although the molecular basis of this is unclear. Here we demonstrate IFN uniquely and specifically ligates to IFNAR1 in an IFNAR2-independent manner and provide the structural basis of the IFNAR1-IFN interaction. We show that the IFNAR1-IFN complex transduces signals to modulate the expression of a set of genes independently of IFNAR2. Moreover, we show the in vivo importance of the IFNAR1-IFN signaling axis in a murine model of LPS-induced septic shock. Thus, we provide a molecular basis for understanding specific functions of IFN?. Interferon b induced gene expression in peritoneal exudate cells was measured 3hr post intra-peritoneal injection of 10,000IU/ml of interferon beta or saline into wildtype and Ifnar2-/- mice. Three independant experiments were performed for each treatment in both genotypes using different mice for each sample.

2013-07-13 | E-GEOD-48827 | biostudies-arrayexpress

Flavivirus antagonism of type I interferon signaling reveals prolidase as a regulator of IFNAR1 trafficking and expression

Project description:Type I interferon (IFN-I) signals through two receptor subunits, IFNAR1 and IFNAR2, to regulate sterile and infectious immunity. IFNAR1 expression is tightly regulated to prevent autoimmunity although the mechanisms governing this are incompletely understood. We investigated the strategies used by two flaviviruses, tick-borne encephalitis virus and West Nile virus, to antagonize IFN-I signaling. Infection with these viruses resulted in depletion of IFNAR1 associated with the function of the viral IFN-I antagonist, NS5. NS5 function was dependent on its ability to associate with prolidase (PEPD), a cellular dipeptidase. PEPD was required for IFNAR1 maturation and accumulation, as well as gene induction following IFNAR1 stimulation. The relevance of PEPD to human biology was confirmed in fibroblasts derived from patients with genetic prolidase deficiency that expressed low IFNAR1 and exhibited reduced responses to IFNAR1. Thus, by understanding flavivirus IFN-I antagonism, PEPD is revealed as a central regulator of IFN-I responses in humans. RNA was isolated from replicates of 4 cultured dermal fibroblast lines derived from patients with genetic prolidase deficiency (PEPD), as well as from 4 cultured dermal fibroblast lines derived from normal healthy donors. These were run on Agilent microarrays to compare differences in gene expression observed in PEPD fibroblasts compared with normal fibroblasts.

2015-07-08 | E-GEOD-64387 | biostudies-arrayexpress

Flavivirus antagonism of type I interferon signaling reveals prolidase as a regulator of IFNAR1 trafficking and expression

2015-07-08 | GSE64387 | GEO

ID8 whole exome sequence

Project description:The data in this submission relate to whole exome sequencing from murine ovarian cancer cell line ID8. All sequencing was performed by Beckman Coulter Genomics, Grenoble, France in February 2013.

2018-03-31 | E-MTAB-4663 | biostudies-arrayexpress

Effect of Ifnar1 Knockdown in a mouse model of Cerebral Interferonopathy

Project description:To investigate effect of antisense oligonucleotides targeting the murine IFNa receptor (Ifnar1 ASOs) in a transgenic mouse model of cerebral interferonopathy (GIFN), mice were injected intracerebroventricularly with Ifnar1 ASOs. We performed DGE-Seq on brain cortex samples to determine the impact of ASOs on the transcriptome. Treatment of GIFN mice with Ifnar1 ASOs resulted in a significant decrease in Interferon gene expression and dampened the hyperinflammatory transcriptomic landscape in the brains of GIFN mice.

2023-12-22 | GSE231627 | GEO

Structural basis of a novel Interferon b signaling axis mediated via the ifnar1 receptor

2013-07-13 | GSE48827 | GEO

RNA-seq analysis of ID8 ovarian cancer cells after harvest from malignant ascites

Project description:Sequencing of mRNA from ID8 tumor cells and ID8 tumor cells harvested from ascites of mice 11 weeks after intra peritoneal inoculation show acquisition of cancer stem cell-like features in ascitic tumor cells.

2020-01-06 | E-MTAB-8140 | biostudies-arrayexpress

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