Project description:Japanese flounder (Paralichthys olivaceus) is an economic important aquaculture fish that was susceptible to Vibrio anguillarum. To fully deciphered the molecular mechanisms underlying flounder host defense against V. anguillarum infection, we perform the micro-transcriptome analysis of founder spleen with and without V. anguillarum infection at 3 time points.
2022-01-12 | GSE143478 | GEO
Project description:Transcriptome analysis of turbot liver with Vibrio anguillarum infection
Project description:The objective of this study was to determine how transcriptomic responses in the spleen and liver are altered in juvenile Micropogonias undulatus exposed to oil, a known fish pathogen (Vibrio anguillarum), or both.
Project description:The objective of this study was to determine how transcriptomic responses in the spleen and liver are altered in juvenile Sciaenops ocellatus exposed to oil, a known fish pathogen (Vibrio anguillarum), or both.
Project description:The objective of this study was to determine how transcriptomic responses in the spleen and liver are altered in juvenile Paralichthys lethostigma exposed to oil, a known fish pathogen (Vibrio anguillarum), or both.
2020-05-06 | GSE149905 | GEO
Project description:Transcriptome analysis of turbot head kidney with Vibrio anguillarum infection
Project description:This experiment aims at mapping the early response of lumpfish leukocytes against Vibrio anguillarum exposure. Fifteen fish were sacrificed and their head kidney were excised. From the tissue samples, leukocytes where isolated. 5x10e6 cells, from each leukocyte sample where added to each experimental sample. As it was 4 experimental groups, a total of 2 * 10e7 cells were used per fish. The experimental groups was control 6 hours, treatment 6 hours, control 24 hours and treatment 24 hours. The control groups were added phosphate buffered saline, while the treatment groups where added 5*10 to the 7th Vibrio anguillarum O1 cells suspended in an equal amount of phosphate buffered saline. After experiment was ended, the cells were lyzed and RNA was extracted, DNase treated, normalized and pooled . The normalization and pooling was conducted by adding 1000 nanograms RNA from 5 experimental samples to one sequencing sample. The sequencing libraries were prepared and sequenced by the Norwegian High Throughput Sequencing Center. . Reads of low quality, low complexity, containing adapter sequence, matching ribosomal or mitochondrial sequence, or the genomes of Vibrio anguillarum used in the stimulation experiment were discarded. Transcripts were assembled using Trinity v2.0.6. Transcript abundances were estimated using RSEM as part of the Trinity pipeline. The read count estimates were used as a basis for differential expression analysis using the Limma R-package. Only genes with at least 10 reads in at least three samples were considered for differential expression analysis.
Project description:The gram-negative bacterium Vibrio (Listonella) anguillarum is the causative agent of vibriosis, a bacterial disease affecting aquacultural industry across the globe. The full mechanism of V. anguillarum pathogenesis is not completely understood, but many virulence determinants have been identified. The current study aimed to obtain molecular insights into the proteome response of the bacterium to several conditions mimicking vibriosis. Our data shed light on the adaptability of V. anguillarum to oxidative stress, iron limitations and the complement system, and offer potential virulence determinants associated in particular with septicemia over the course of vibriosis.
Project description:The objective of this study was to determine how transcriptomic responses in the spleen are altered in juvenile red snapper exposed to oil, a known fish pathogen (Vibrio anguillarum), or both.
