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Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer

ABSTRACT: Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer

PROVIDER: PRJNA890182 | ENA |

REPOSITORIES: ENA

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Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer [scRNA-Seq]

Project description:Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer [scRNA-Seq]

| PRJNA890193 | ENA

Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer [RNA-Seq]

Project description:Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer [RNA-Seq]

| PRJNA890194 | ENA

Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer [scRNA-Seq]

Project description:Pancreatic ductal adenocarcinoma (PDAC) is characterized by rich deposits of extracellular matrix (ECM), affecting the pathophysiology of the disease. Here, we identified galectin 4 (gal 4) as a cancer cell produced protein deposited into the ECM of PDAC tumors and detected high circulating levels of gal 4 in PDAC patients. In orthotopic transplantation experiments we observed increased infiltration of T-cells and prolonged survival in immunocompetent mice transplanted with cancer cells with reduced expression of gal 4. Increased survival was not observed in immunodeficient RAG1-/- mice, demonstrating that the effect was mediated by the adaptive immune system. Furthermore, by performing single-cell RNA-sequencing we found that the myeloid compartment and cancer-associated fibroblast (CAF) subtypes were altered in the transplanted tumors. Reduced gal 4 expression was associated with higher proportion of myofibroblastic CAFs and reduced numbers of inflammatory CAFs. We also found higher proportions of M1 macrophages, T-cells and antigen presenting dendritic cells in tumors with reduced gal 4 expression. Using a co-culture system, we observed that extracellular gal 4 induced apoptosis in T-cells by binding CD3, resulting in anergy or induction of apoptosis. Hence, we show that gal 4 is involved in immune evasion and identify gal 4 as a promising drug target for overcoming immunosuppression in PDAC.

2022-10-18 | GSE215388 | GEO

Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer [RNA-Seq]

2022-10-18 | GSE215387 | GEO

Lactate sensing drives fungal immune evasion

Project description:Lactate sensing drives fungal immune evasion

| PRJEB13396 | ENA

Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-10-18 | GSE215389 | GEO

A tumor specific super-enhancer drives immune evasion by guiding synchronous expression of PD-L1 and PD-L2

Project description:A tumor specific super-enhancer drives immune evasion by guiding synchronous expression of PD-L1 and PD-L2

2019-07-30 | GSE135016 | GEO

Galectin-3 regulates the expression of various genes in human gastric cancer cells

Project description:Although galectin-3 is known to modulate the cell proliferation, RNA processing, tumorigenesis, metastasis and apoptosis and also, which is highly expressed in human cancers, the function of galectin-3 is still controversy in gastric cancer. Here, we demonstrated the function of galectin-3 on gastric cancers by silencing with synthetic double-stranded small interfering RNA (siRNA). After silencing of galectin-3, cell numbers decreased and cell shapes changed in round. To determine the mechanism, microarray analysis was used to detect the changes in gene expression by galectin-3 silencing.

2011-05-31 | GSE29630 | GEO

Galectin-3 regulates the expression of various genes in human gastric cancer cells

2011-05-31 | E-GEOD-29630 | biostudies-arrayexpress

Innate immune function of galectin-1 as a DAMP

Project description:Galectin-1 is a prototype member of the galectin family of b-galactoside-binding proteins with several immunoregulatory activities (Sundblad et al., 2017). Although this lectin exerts extracellular activities by cross-linking cell surface glycoconjugates, the mechanisms and molecular machinery involved in its secretion are uncertain as it lacks the classical signal sequence required for ER-Golgi release (Camby, 2006; Croci et al., 2014). To assess the global impact of galectin-1 on LPS-induced transcriptomic response, we performed RNAseq analysis of total RNAs from the spleen and the lungs of wild-type and Lgals1-/- mice injected with LPS.

2020-10-29 | GSE140892 | GEO

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